Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Production of P(3-hydroxybutyrate-3-hydroxyvalerate) and P(3-hydroxybutyrate-4-hydroxybutyrate) Using Transformant Alcaligenes latus Enforcing Its Own phbC Gene

  • Seo, Il-Seon (Department of Genetic Engineering, College of Natural Sciences, Kyungpook National University) ;
  • Jung, Young-Mi (Department of Genetic Engineering, College of Natural Sciences, Kyungpook National University) ;
  • Lee, Yong-Hyun (Department of Genetic Engineering, College of Natural Sciences, Kyungpook National University)
  • Published : 2001.04.01
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Abstract

An isolated phbC gene from Alcaligenes latus was reintroduced into the parent A. latus through the transformation process, and the effect of the amplified phbC gene on the biosynthesis of P(3-hydroxybutyrate-3-hydroxyvalerate) [P(3HB-3HV)] and P(3-hydroxybutyrate-4-hydroxybutyrate) [P(3HB-4HB)] in the transformant A. latus was investigated. The biosynthesis rate and content of the above copolymers increased up to 1.3-fold after enforcing its own phbC gene, and the molar fractions of 3HV and 4HB in P(3HB-3HV) and P(3HB-4HB) also changed remarkably from 35.0 to 48.0% and from 34.0 to 56.0%, respectively, showing a critical role of PHB synthase which catalyzes the polymerizing reactions between eiher 3HV or 4HB from precursor compounds and 3HB.

Keywords

References

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