Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Enumeration of Vibrio vulnificus in Natural Samples by Colony Blot Hybridization

  • Lee, Jeong-Hyun (Department of Food Science and Technology, Institute of Biotechnology, Chonnam National University) ;
  • Lee, Kyu-Ho (Department of Environmental Science, Hankuk University of Foreign Studies) ;
  • Choi, Sang-Ho (Department of Food Science and Technology, Institute of Biotechnology, Chonnam National University)
  • Published : 2001.04.01
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Abstract

Colony blot hybridization using a VVHP DNA probe derived from the sequence of the hemolysin gene, vvhA, was specific in identifying all V. vulnificus strains, thereby, eliminating the need for any additional phenotypic identification. The colony blot hybridization procedure revealed a sensitivity and broad applicability sufficient for the direct enumeration of V. vulnificus in various natural samples, without the use of enrichment or culturing on selective medis. V. vulnificus was detected in all natural samples collected during August and May at concentrations ranging from $2.1{\times}10^1\;to\;4.0{\times}10^3$ organisms per ml. However, during November and February, when the mean temperatures of the seawater were $12^{\circ}C$ and $5^{\circ}C$, respectively, V. vulnificus was not detected in any natural samples.

Keywords

References

  1. Appl. Environ. Microbiol. v.61 Nested PCR method for rapid and sensitive detection of Vibrio vulnificus in fish, sediments, and water Arias, C. R.;E. Garay;R. Aznar
  2. Annu. Rev. Microbiol. v.34 Diseases of humans (other than cholera) caused by Vibrios Blake, P. A.;R. E. Weaver;D. G. Hollis
  3. Appl. Environ. Microbiol. v.57 Use of the polymerase chain reaction in detection of culturable and nonculturable Vibrio vulnificus cells Brauns, L.;M. C. Hudson;J. D. Oliver
  4. J. Food Technol. v.6 Food safety applications of nucleic acid-based assays Bsat, N.;M. Wiedmann;J. Czajka;F. Barany;M. Piani;C. A. Batt
  5. Appl. Environ. Microbiol. v.58 Development of monoclonal antibodies that identify Vibrio species commonly isolated from infections of humans, fish, and shellfish Chen, D.;P. J. Hanna;K. Altman;A. Smith;P. Moon;L. S. Hammond
  6. Appl. Environ. Microbiol. v.62 Detection of Bibrio vulnificus biotypes 1 and 2 in eels and oyster by PCR amplication Coleman, S. S.;D. M. Melanson;E. G. Biosca;J. D. Oliver
  7. Appl. Environ. Microbiol. v.60 Densities of Vibrio vulnificus in the intestines of fish from the U. S. Gulf coast DePaola, A.;G. M. Capers;D. Alexander
  8. Anal. Biochem. v.132 A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity Feinberg, A. P.;B. Vogelstein
  9. Appl. Environ. Microbiol. v.59 Enumeration of Vibrio vulnificus on membrane filters with a fluorescently labeled oligonucleotide probe specific for kingdom-level 16S rRNA sequence Heidelberg, J. F.;K. R. O'Neill;D. Jacobs;R. R. Colwell
  10. Appl. Environ. Microbiol. v.57 Polymerase chain reaction identification of Vibrio vulnificus in artificaially contaminated oysters Hill, W. E.;S. P. Keasler;M. W. Trucksess;P. Feng;C. A. Kaysner;K. A. Lampel
  11. Dictionary of Japanese Fish Name and Their Foreign Edquivalents Ichthyological Society of Japan
  12. Korean J. Molacol. v.1 no.Sup. Korean names of molluscs in Korea Je, J. G.
  13. J. Microbiol. Biotechnol. v.9 Intraspecific variation of environmental and clinical Vibrio vulnificus isolates as demonstrated by restriction endonuclease digestion profiles Kim, K. Y.;H. C. Yang;M. L. Tamplin;S. H. Choi
  14. J. Food Sci. v.62 Improving detection of Vibrio vulnificus in Octopus variabilis by PCR Lee, J. Y.;J. B. Eun;S. H. Choi
  15. J. Food Sci. v.64 Two-stage nested PCR effectiveness for direct detection of Vibrio vulnificus in natural samples Lee, J. Y.;Y. B. Bang;J. H. Rhee;S. H. Choi
  16. Appl. Environ. Microbiol. v.53 New selective and differential medium for Vibrio cholerae and Vibrio vulnificus Massad, G.;J. D. Oliver
  17. Appl. Environ. Microbiol. v.59 Direct plating procedure for enumerating Vibrio vulnificus in oysters (Crassostrea virginica) Miceli, G. A.;W. D. Watkins;S. R. Rippey
  18. Methods Enzymol. v.57 Isolation, identification, and manipulation of luminous bacteria Nealson, K. H.
  19. Food Microbiology Fundamentals and Frontiers Vibrio species Oliver, J. D.;J. B. Kaper;Doyle, M. P.(ed.);Beuchat, L. R.(ed.);Montville, T. J.(ed.)
  20. Appl. Enciron. Microbiol. v.45 Distribution of Vibrio vulnificus and other lactose-fermenting Vibrios in the marine environment Oliver, J. D.;R. A. Warner;D. L. Cleland
  21. Appl. Environ. Microbiol. v.57 Formation of nonculturable Vibrio vulnificus cells and its relationship to the starvation state Oliver, J. D.;L. Nilsson;S. Kjelleberg
  22. Appl. Environ. Microbiol. v.58 Use of colistin-polymixin B-cellobiose agar for iosolation of Vibrio vulnificus from the environment Oliver, J. D.;K. Guthrie;J. Preyer;A. Wright;L. M. Simpson;R. Siebeling;J. G. Morris, Jr.
  23. Appl. Environ. Microbiol. v.61 Entry into, and resuscitation from, the viable but nonculturable state by Vibrio vulnificus in an estuarine environment Oliver, J. D.;F. Hite;D. McDoulgad;M. L. Andon;L. M. Simpson
  24. Appl. Environ. Microbiol. v.58 Seasonal incidence of Vibrio vulnificus in the Great Bay estuary of New Hampshire and Maine O'Neill, K. R.;S. H. Jones;D. J. Grimes
  25. Microbiol. Rev. v.51 Survival strategies of bacteria in the natural environment Roszak, D. B.;R. R. Colwell
  26. Science v.239 Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase Saiki, R. K.;D. H. Garland;S. Stoffel;S. J. Scharf;R. Higuchi;G. T. Horn;K. B. Mullis;H. A. Erlich
  27. Molecular Cloning: A Laboratory Manual(2nd ed.) Sambrook, J.;E. F. Fritsch;T. Maniatis
  28. Curr. Opin. Biotechnol. v.3 Methods of microbial identification, tracking and monitoring of function Stahl, D. A.;M. D. Kane
  29. J. Infect. Dis. v.149 Clinical features and an epidemiological study of vibrio vulnificus infections Tacket, C. O.;F. Brenner;P. A. Blake
  30. Appl. Environ. Microbiol. v.57 Enzyme immunoassay for identification of Vibrio vulnificus in seawater, sediment, and oysters Tamplin, M. L.;A. L. Martin;A. D. Ruple;D. W. Cook;C. W. Kaspar
  31. Appl. Environ. Microbiol. v.44 Isolation and characterization of Vibrio vulnificus from two Florida estuaries Tamplin, M. L.;G. E. Rodrick;N. J. Blake;T. Cuba
  32. Appl. Environ. Microbiol. v.62 Distribution of Vibrio vulnificus in the Chesapeake Bay Wright, A. C.;R. T. Hill;J. A. Johnson;M. C. Roghman;R. R. Colwell;J. G. Morris, Jr.
  33. Appl. Environ. Microbiol. v.59 Rapid identification of Vibrio vulnificus on nonselective media with an alkaline phosphatase-labeled oligonucleotide probe Wright, A. C.;G. A. Miceli;W. L. Landry;J. B. Christy;W. D. Watkins;J. G. Morris, Jr.
  34. Infect. Immun. v.59 The extracellular cytolysin of vibrio vulnificus: Inactivation and relationship to virulence in mice Wright, A. C.;J. G. Morris, Jr.
  35. Infect. Immun. v.50 Cloning of the cytotoxin-hemolysin gene of Vibrio vulnificus Wright, A. C.;J. G. Morris, Jr.;D. R. Maneval, Jr.;K. Richardson;J. B. Kaper
  36. Infect. Immun. v.58 The cytolysin gene of Vibrio vulnificus: Sequence and relationship to the Vibrio cholera El Tor hemolysin gene Yamamoto, K.;A. C. Wright;J. B. Kaper;J. G. Morris, Jr.