Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Cloning of the $Glu-tRNA^{Gln}$ Amidotransferase (gatCAB) Gene from Staphylococcus aureus

  • Namgoong, Suk (Graduate School of Biotechnology, Korea University) ;
  • Hong, Kwang-Won (Department of Food Science and Technology, Dongguk University) ;
  • Lee, Se-Yong (Graduate School of Biotechnology, Korea University)
  • Published : 2001.04.01
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Abstract

In Gram(+) bacteria and organelles in higher eukarotes, $Gln-tRNA^{Gln}$ utilized for protein biosynthesis is formed by a tRNA-dependent amino acid transformation using mischarged $Gln-tRNA^{Gln}$ as the intermediate. In this study, the gatCAB gene encoding $Gln-tRNA^{Gln}$ amidotransferase (Glu-AdT) of Staphylococcus aureus was cloned and its nucleotide sequence wa determined. The S. aureus gatCAB gene was organized in an operon structure consisting of three open reading frames (gatC, gatA, and gatB), similar to that of Bacillus subtilis. The gene sequences for the A and B subunits of$Gln-tRNA^{Gln}$ amidotransferase showed significant homology (77 and 87% homology with amino acid sequence) with the gatA and gatB genes of B. subtilis, yet the C subunit (gatC) showed a relatively lowe homology with the B. subtilis gatC gene and other orthologues. The cloned S. aureus <$Gln-tRNA^{Gln}$ amidotransferase gene was highly expressed in Escherichia coli, and the resulting crude enzyme could convert misacylated <$Gln-tRNA^{Gln}$ into $Gln-tRNA^{Gln}$ in vitro.

Keywords

References

  1. Staphylococci and Staphylococcal Disease v.1 Veterinary aspects of staphylococci Anderson, J. C.
  2. Proc. Natl. Acad. Sci. USA v.95 Thermus thermophilus: A link in evolution of the tRNA-dependent amino acid amidation pathways Becker, H. D.;D. Kern
  3. Microbiology v.142 The 52 degrees-55 degrees segment of the Bacillus subtilis chromosome: A region devoted to purine uptake and metabolism, and containing the genes cotA, gobP and guaA and the pur gene cluster within a 34,960 bp nucleotide sequence Borriss, R.;S. Porwollik;R. Schroeter
  4. Proc. Natl. Acad. Sci. USA v.95 Glu-AdT: A novel heterotrimeric enzyme required for correct decoding of glutamine codons during translation Curnow, A. W.;K. W. Hong;R. Yuan;S. I. Kim;O. Martins;W. Winkler;T. M. Henkin;D. Soll
  5. Nucleic Acids Symp. Ser. v.36 tRNA-dependent amino acid transformations Curnow, A. W.;K. W. Hong;R. Yuan;D. Soll
  6. Proc. Natl. Acad. Sci. USA v.95 Glutamyl-tRNA(Gln) amidotransferase in Deinococcus radiodurans may be confined to asparagine biosynthesis Curnow, A. W.;D. L. Tumbula;J. T. Pelaschier;B. Min;D. Soll
  7. J. Biol. Chem. v.248 The distribution of glutaminase isoenzymes in the various structures of the nephron in normal, acidotic, and alkalotic rat kidney Curthoys N. P.;O. H. Lowry
  8. Microbiol. Mol. Biol. Rev. v.63 Helicobacter pylori physiology predicted from genomic comparison of two strains Doig, P.;B. L. de Jonge;R. A. Alm;E. D. Brown;M. Uria-Nickelsen;B. Noonan;S. D. Mills;P. Tummino;G. Carmel;B. C. Guild;D. T. Moir;G. F. Vovis;T. J. Trust
  9. J. Biol. Chem. v.271 Widespread use of the glu-tRNAGln transamidation pathway among bacteria. A member of the alpha purple bacteria lacks glutaminyl-tRNA synthetase Gagnon, Y.;L. Lacoste;N. Champagne;J. Lapointe
  10. J. Bacteriol. v.175 Staphylococcus aureus has clustered tRNA genes Green, C.;B. Vold
  11. J. Bacteriol. v.174 Analysis of the Bacillus subtilis tyrS gene: Conservation of a regulatory sequence in multiple tRNA synthetase genes Henkin, T. M.;B. L. Glass;F. J. Grundy
  12. Methods of Enzymology v.266 Using CLUSTAL for multiple sequence alignments Higgins, D. G.;J. D. Thompson;T. J. Gibson
  13. J. Biol. Chem. v.265 Purification and functional characterization of the Glu-tRNA(Gln) anidotransferase from Chlamydomonas reinhardtii Jahn, D.;Y. C. Kim;Y. Ishino;M. W. Chen;D. Soll
  14. J. Bacteriol. v.179 Purification and functional characterization of the Glu-tRNA(Gln) anidotransferase from Chlamydomonas reinhardti Kim, S. I.;N. Stange-Thomann;O. Martins;K. W. Hong;D. Soll;T. D. Fox
  15. J. Microbiol. Biotechnol. v.6 Relationship between two tetracycleine resistance plasmids of Staphylococcus aureus in Korea Kim, W.-K.;C. K. Shin;K. H. Moon
  16. J. Microbiol. Biotechnol. v.9 Multimeric expression of the antimicrobial peptide buforin Ⅱ in Escherichia coli by fusion to a cysteine-rich acidic peptide Lee, J. H.;J. H. Kim;S. S. Hong;H. S. Lee;S. C. Kim
  17. EMBO J. v.18 Aminoacyl-tRNA synthetases: A family of expanding functions Martinis, S. A.;P. Plateau;J. Cavarelli;C. Florentz
  18. Biosynthesis and Function tRNA: Structure Meinnel, L. L.;Y. Mechulam;S. Blanquet;Soll, D.(ed.);RajBhandary, U.(ed.)
  19. Nucleic Acids Res. v.26 CyanoBase, a www database containing the complete nucleotide sequence of the genome of Synechocystis sp. strain PCC6803 Nakamura, Y.;T. Kaneko;M. Hirosawa;N. Miyajima;S. Tabata
  20. Proc. Natl. Acad. Sci. USA v.94 Once there were twenty RajBhandary, U. L.
  21. Proc. Natl. Acad. Sci. USA v.94 DNA sequencing with chain-terminating inhibitors Sanger, F.;S. Nicklen;A. R. Coulson
  22. FASEB J. v.12 Aminoacyl tRNA synthetases as targets for new anti-infectives Schimmel, P.;J. Tao;J. Hill
  23. Biochimie v.70 Misaminoacylation and transamidation are required for protein biosynthesis in Lactobacillus bulgaricus Schon, A.;H. Hottinger;D. Soll
  24. Nature v.331 Protein biosynthesis in organelles requires misaminoacylation of tRNA Schon, A.;C. G.;Kannangara;S. Gough;D. Soll
  25. J. Bacteriol. v.170 Characterization of the glutamyl-tRNA(Gln)-to-glutaminyl-tRNA(Gln) amidotransferase reaction of Bacillus subtilis Strauch, M. A.;H. Zalkin;A. I. Aronson
  26. Nature v.388 The complete genome sequence of the gastric pathogen Helicobacter pylori Tomb, J. F.;O. White, A. R.;Kerlavage;R. A. Clayton;G. G. Sutton;R. D. Fleischmann;K. A. Ketchum;H. P. Klenk;S. Gill;B. A. dougherty;K. Nelson;J. Quackenbush;L. Zhou;E. F. Kirkness;S. Peterson;B. Loftus;D. Richardson;R. Dodson;H. G. Khalak;A. Glodek;K. McKenney;L. M. Fitzegerald;N. Lee;M. D. Adams;J. C. Venter
  27. Appl. Environ. Microbiol. v.61 Identification of a PutP proline permease gene homolog from Staphylococcus aureus by expression cloning of the high-affinity proline transport system in Escherichia coli Wengender, P. A.;K. Miller
  28. Proc. Natl. Acad. Sci. USA v.61 Transfer RNA as a cofactor coupling amino acid synthesis with that of protein Wilcox, M.;M. Nirenberg