Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Enhanced Transformation Efficiency of an Anticoagulant Hirudin Gene into Saccharomyces cerevisiae by a Double ${\delta}-Sequence$

  • Kim, Myoung-Dong (Department of Food Science and Technology, Seoul National University) ;
  • Yoo, Young-Je (School of Chemical Engineering, Seoul National University) ;
  • Rhee, Sang-Ki (Korea Research Institute of Bioscience and Biotechnology) ;
  • Seo, JIn-Ho (Department of Food Science and Technology, Seoul National University)
  • Published : 2001.02.01
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Abstract

Delta-integration vectors were constructed for the purpose of achieving homologous integration of the hirudin expression cassette into the chromosome of Saccharomyces cerevisiae. A double $\delta$ system truncated with the unnecessary bacterial genes, and consequently having a reduced insert size for integration, showed a four-fold increase in transformation efficiency at given DNA concentrations, and as a result, the constructed recombinant yeast strain had a 1.3-fold enhancement in hirudin expression level compared with a single $\delta$ system.

Keywords

References

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