Reduced Susceptibility of a Model Saccharomyces cerevisiae Biofilm to Osmotic Upshifts

  • Jirku Vlacimir (Department of Fermentation Chemistry and Bioengineering, Institute of Chemical Technology) ;
  • Jan Masak (Department of Fermentation Chemistry and Bioengineering, Institute of Chemical Technology) ;
  • Alena Cejkova (Department of Fermentation Chemistry and Bioengineering, Institute of Chemical Technology)
  • Published : 2001.02.01

Abstract

Whole-cell attachment by covalent linkage, thereby simulating natural and specific attachments, improves the osmotolerance of Saccharomyces cerevisiae cells. The enhanced osmoresistance is correlated with a decrease in the intercellular concentration of trehalose and accompanied by membrane compositional changed. The results obtained indicate that yeast cell-support (physical) contact is sensed and responded to.

Keywords

References

  1. Can. J. Biochem. Physiol. v.37 A rapid method of total lipid extraction and purification Bligh, E. G.;W. J. Dyer
  2. Adv. Microb. Physiol. v.33 Physiology of osmotolerance in fungi Blomberg, A.;L. Adler
  3. Science v.259 An osmosensing signal transduction pathway in yeast Brewster, J. L.;T. de Valoir;N. D. Dweyer;E. Winter;M. C. Gustin
  4. J. Appl. Microbiol. v.88 Reduced suscptibility of thin Pseudomonas aeruginosa biofilms to hydrogen peroxide and monochloramine Cochran, W. L.;G. A. McFeters;P. S. Stewart
  5. Microbiol. v.144 Role of trehalose in survival of Saccharomyces cerevisiae under osmotic stress Hounsa, C. G.;E. V. Brandt;J. Thevelein;S. Hohman;B. A. Prior
  6. World J. Microbiol. Biotechnol. v.8 Nystatin and killer toxin sensitivity of free and immobilized Saccharomyces cerevisiae Jirku, V.
  7. Experientia v.51 Covalent immobilization as a stimulus of cell wall composition changes Jirku, V.
  8. World J. Microbiol. Biotechnol. v.11 Novel way to study the chronological changes in yeast outer-wall proteins Jirku, V.
  9. J. Chem. Tech. Biotechnol. v.65 The use of covalently immobilized mycelia to modify the β-glucanase system of Aspergillus vesicolor Jirku, V.
  10. Proc. ISER '97 The use of attached cells to modify the cell sensitivity to xenobiotics affecting biological membrane Jirku, V.;J. Masak;A. Cejkova;H. Verachtert(ed.);W. Verstraete(ed.)
  11. Enzyme Microbiol. Technol. v.26 Yeast cell attachment: A tool modulating wall composition and resistance to 5-bromo-6-azauracil Jirku, V.;J. Masak;A. Cejkova
  12. Meth. Enzymol. v.135 Cell immobilization by covalent linkage Jirku, V.;J. Turkova
  13. Biol. Rev. v.55 Regulation of contact behaviour of cells Jones, B. M.
  14. Cancer Metastasis Rev. v.14 Adhesion or anti-adhesion in cancer:What matters more? Jothy, S.;S. B. Munro;L. LeDuy;D. McClure;O. W. Blaschuk
  15. Laboratory Techniques in Biochemistry Nitrogen quantitative assay Keil, B.;A. Kleinzeller;B. Keil(ed.);Z. Sormova(ed.)
  16. Appl. Environ. Microbiol. v.59 Role of growth phase and ethanol in freeze-thaw stress resistance of Saccharomyces cervisiae Lewis, J. G.;R. P. Learmonth;K. Watson
  17. Bacterial Adhesion Coaggregation:Enhancing colonization in a fluctuation environment London, J.;P. E. Kolenbrander;M. Fletcher(ed.)
  18. Nature v.369 A two component system that regulates an osmosensing MAP kinase cascade in yeast Maeda, T.;S. M. Wurgler-Murphy;H. Saito
  19. FEMS Microbiol. Rev. v.21 Osmoregulation and glycerol metabolism in the yeast Saccharomyces cerevisiae Nevoigt, E.;U. Stahl
  20. J. Food. Technol. v.1 An equation for the activity and equilibrium relative humidities of water in confectionary syrups Norrish, R. S.
  21. Microbiologia Sem. v.7 On the role of trehalose in yeast cells subjected to hyperosmotic shock Pardo, C.;M.A. Lapena;M. Gasto
  22. J. Biotechnol. v.36 The use of epifluorescence to determine the viability of Saccharomyces cerevisiae subjected to osmotic shifts Raynal, L.;P. Barnwell;P. Gervais
  23. Meth. Enzymol. v.111 High-performance liquid chromatography of sterols: Yeast sterols Rodrigues, R. J.;L. W. Parks
  24. Int. Rev. Cytol. v.165 Salt tolerance in plants and microorganisms:Toxicity targets and defense responses Serrano, R.
  25. Annu. Rev. Cytol. v.165 Thinking about bacterial population as multicellular organisms Shapiro, J. A.
  26. FEMS Microbiol. Lett. v.168 Stress tolerance in a yeast sterol auxotroph: Role of ergosterol, heat shock proteins and trehalose Swan, T. M.;K. Watson
  27. Folia Microbiol. v.40 Influence of ethanol on the lipid content and fatty acid composition of Saccharomyces cerevisiae Sajbidor, J.;Z. Ciesarova;D. Smogrovicova
  28. Microbiol. Mol. Rev. v.63 Osmosensing by bacteria: Signals and membrane-based sensors Wood, J. M.