Biotechnology and Bioprocess Engineering:BBE

  • 제6권3호
  • /
  • Pages.200-204
  • /
  • 2001
  • /
  • 1226-8372(pISSN)
  • /
  • 1976-3816(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
권호 표지

Concentration of 6-Amonopenicillanic Acid from Penicillin Bioconversion Solution and Its Mother Liquor by Nanofiltration Membrane

  • Xuejun Cao (State Key Laboratory of Bioreactor Engineering, Department of Biochemical Engineering, East China University of Science and Technology) ;
  • Wu, Xing-Yan (State Key Laboratory of Bioreactor Engineering, Department of Biochemical Engineering, East China University of Science and Technology) ;
  • Tong Wu (Millipore China Coporation Limited) ;
  • Keming Jin (Millipore China Coporation Limited) ;
  • Hur, Byung-Ki (Department of Biological Engineering, Inha University)
  • 발행 : 2001.05.01
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초록

In this study, nanofiltration was applied to the concentration of the 6-aminopenicillinic acid (6-APA) from bioconverted penicillin solution and also to its mother liquor. The 6-APA in the solution was concentrated from 0.211 mol/L to 0.746 mol/L by nanofiltration. The final maximum concentration was 3.6 times higher than the initial concentration an the recovery yield was 97% to 99% of the original 6-APA. The concentrated solution was crystallized with the yields of 88.9-90.2% and the purity of the crystallized product was about 98%. The concentration of 6-APA in the mother liquor after crystallization was 0.014 mol/L and thus was concentrated 20-30 fold by nanofiltration and crystallization. The recovery of 6-APA was over 98%. The salts contained in the mother liquor, such as NH$_4$Cl and KCl, could be removed by allowing them to permeate through the membrane.

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참고문헌

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