Biotechnology and Bioprocess Engineering:BBE

  • 제6권3호
  • /
  • Pages.179-182
  • /
  • 2001
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  • 1226-8372(pISSN)
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  • 1976-3816(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
권호 표지

Polyacrylamide Gel Immobilization of Porcine Liver Esterase for the Enantioselective Production of Levofloxacin

  • Lee, Sang-Yoon (Department of Biological Engineering and Center for Advanced Bioseparation Technology, Inha University) ;
  • Min, Byung-Hyuk (Department of Biological Engineering and Center for Advanced Bioseparation Technology, Inha University) ;
  • Song, Seong-Won (Boryung Central Research Institute, Boryung Pharmaceutical Co.Ltd.) ;
  • Oh, Sun-Young (Boryung Central Research Institute, Boryung Pharmaceutical Co.Ltd.) ;
  • Lim, Sang-Min (Boryung Central Research Institute, Boryung Pharmaceutical Co.Ltd.) ;
  • Kim, Sang-Lin (Boryung Central Research Institute, Boryung Pharmaceutical Co.Ltd.) ;
  • Kim, Dong-Il (Department of Biological Engineering and Center for Advanced Bioseparation Technology, Inha University)
  • 발행 : 2001.05.01
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초록

Porcine liver esterase was immobilized in polyacrylamide gel for the enantioselective production of levofloxacin from ofloxacin butyl ester. The initial activity of immobilized esterase was found to be significantly affected by the polyacrylamide gel composition. The optimum concentrations of monomer and crosslinker were determined to be 20% and 8.3%, respectively. The activity of immobilized esterase was 55.4% compared to a free enzyme. Enantiomeric excess was maintained at 60%, almost the same level as that of free enzyme. In addition, the immobilized esterase could be used repeatedly up to 10 times without experiencing any severe loss of activity and enantioselectivity.

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