Biotechnology and Bioprocess Engineering:BBE

  • 제6권3호
  • /
  • Pages.173-178
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  • 2001
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  • 1226-8372(pISSN)
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  • 1976-3816(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
권호 표지

Expression of Nutritionally Well-balanced Protein, AmA1, in Saccharomyces cerevisiae

  • Kim, Tae-Geum (Institute of Molecular Biology and Genetics, Division of Biological Sciences, Chonbuk National University) ;
  • Kim, Ju (Institute of Molecular Biology and Genetics, Division of Biological Sciences, Chonbuk National University) ;
  • Kim, Dae-Hyuk (Institute of Molecular Biology and Genetics, Division of Biological Sciences, Chonbuk National University) ;
  • Yang, Moon-Sik (Institute of Molecular Biology and Genetics, Division of Biological Sciences, Chonbuk National University)
  • 발행 : 2001.05.01
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초록

Food yeast, Saccharomyces cerevisiae, is a safe organism with a long history of use for the production of biomass rich in high quality proteins and vitamins. AmA1, a seed storage albumin from Amaranthus hypochondriacus, has a well-balanced amino acid composition and high levels of essential amino acids and offers the possibility of further improving food animal feed additives. In order to find an effective means of expressing AmA1 in yeast, the gene was cloned into an episomal shuttle vector. Four different promoters were tested: the glyceraldehyde-3-phosphate dehydrogenase promoter, galactose dehydrogenase 10 promoter, alcohol dehydrogenase II promoter, and a hybrid ADH2-GPD promoter. The recombinant AmA1 genes were then introduced into the yeast Saccharomyces cerevisiae 2805. Northern and Western blot analyses of the yeast under appropriate conditions revealed that AmA1 was expressed by all four promoters at varying levels. An enzyme-linked immunosorbent assay demonstrated that the amount of AmA1 protein in the recombinant yeast was 1.3-4.3% of the total soluble proteins. The highest expression level was obtained from the hybrid ADH2-GPD promoter.

키워드

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