Biotechnology and Bioprocess Engineering:BBE

  • 제6권1호
  • /
  • Pages.56-60
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  • 2001
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  • 1226-8372(pISSN)
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  • 1976-3816(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
권호 표지

Improved Degradation of 4-Chlorobiphencyl, 2,3-Dihydroxybiphenyl, and Catecholic Compounds by Recombinant Bacterial Strains

  • Kim, Ji-Young (Department of Microbiology and Biotechnology, and Research Institute for Genetic Engineering, Chungbuk National University) ;
  • Kim, Youngsoo (College of Pharmacy, Chungbuk National University) ;
  • Lee, Kyoung (Department of Microbiology, Changwon National University) ;
  • Kim, Chi-Kyung (Department of Microbiology and Biotechnology, and Research Institute for Genetic Engineering, Chungbuk National University)
  • 발행 : 2001.01.01
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초록

The pcbC gene encoding (4-chloro-)2,3-dihydroxybiphenyl dioxygenase was cloned from the genomic DNA of Pseudomonas sp. P20 using pKT230 to construct pKK1. A recombinant strain, E. coli KK1, was selected by transforming the pKK1 into E. coli XL1-Blue. Another recombinant strain, Pseudomonas sp. DJP-120, was obtained by transferring the pKK1 of E. coli KK1 into Pseudomonas sp. DJ-12 by conjugation. Both recombinant strains showed a 23.7 to 26.5 fold increase in the degradation activity to 2,3-dihydroxybiphenyl compared with that of the natural isolate, Pseudomonas sp. DJ-12. The DJP-120 strain showed 24.5, 3.5, and 4.8 fold higher degradation activities to 4-chlorobiphenyl, catechol, and 3-methylcatechol than DJ-12 strain, respectively. The pKK1 plasmid of both strains and their ability to degrade 2,3-dihydroxybiphenyl were stable even after about 1,200 generations.

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참고문헌

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