KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
권호 표지

Isolation of Bacillus sp. AIR-5 PRoducing Maltopentaose Forming Amylase and Optimization of Maltopentaose Production

Maltopentaose 생산 균의 분리 및 생산 조건 연구

  • 김영민 (전남대학교 의공학협동과정) ;
  • 서은성 (물질·생물화학공학과) ;
  • 김도만 (화학공학부) ;
  • 김도원 (강릉대학교 동해안해양생물자원연구센터) ;
  • 이진하 (일본 홋카이도대학교) ;
  • Published : 2001.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

We isolated a bacterium that produces an extracellular maltopentaose(G5)-forming amylase from amylose and soluble starch. The bacterium was identified and assigned as a Bacillus sp. AIR-5. The amylase did not hydrolyze maltose, maltotriose, maltotetraose or maltopentaose. Optimum medium composition for maltopentaose production in flask culture was 2%(w/v) soluble starch, 0.4%(w/v) tryptone, 0.5%(w/v) NaCl, 0.5%(w/v) K$_2$HPO$_4$, and 3 mM CaCl$_2$at pH 8.0, 28$^{\circ}C$. The highest yield for maltopentaose production in this condition was 6.45 g/L and was 32.55% of theoretical yield.

Keywords

References

  1. Appl. Environ. Microbiol. v.63 Cloning sequencing, and expression of the gene encoding extracellular α-amylase from Pyococcus furiosus and biochemical characterization of the recombinat enzyme Guoqiang, D.;C. Vielle;A. Savchenko;J. Gregory Zeikus
  2. Appl. Environ. Microbiol. v.63 Purification, characterization, and nucleotide sequence of an intracellular maltotriose-producing α-amylase from Streptococcus bovis 148 Eiichi, S.;T. Uchimura;T. Kudo;K. Komagata
  3. The Annual Meeting of the Agri-cultural Chemical Society of Japan Abstracts of Papers Takasaki, Y.
  4. Arch. Biochem. Biophys. v.145 Isolation, purification and characterization of a maltotetraose-producing amylase form Pseudomonas stutzeri Robyt, J. F.;R. J. Ackerman
  5. Arch. Biochem. Biophys. v.155 A thermophilic extracellular α-amylase from Bacillus licheniformis Saito, N.
  6. Agr. Biol. Chem. v.49 Characterization of maltopentaose-producing bacterium and its cultural conditions Yoshigi, N.;T. Chikano;M. Kamimura
  7. Agr. Biol. Chem. v.46 Production of maltohexaose by α-amylase from Bacillus circulans G-6 Takasaki, Y.
  8. Microbial Polysaccharide and Polysaccharides The application of polysaccharide degrading enzymes in the starch industry Norman, B. E.;Berkelely R. C. W.(ed.);Gooday, G. W.(ed.);Ellwood, D. C.(ed.)
  9. Enz. Microbial Technol. v.24 A thermostable α-amylase producing maltohexaose from a new isolated Bacillus sp. US100: study of activity and molecular cloning of the corresponding gene Mamdoub, B. A.;M. Mezghani;S. Bejar
  10. Korean J. Food Sci. Technol. v.13 The effects of addition of oligosaccharide on the quality characteristic of tomato jam Kim, K. S.;Y. K. Chae
  11. Korean J. Food Sci. Technol. v.27 Physical and physiological properties of isomaltooligosaccharides and fructooligosaccharides Kim, J. R.;C. Yook;H. K. Kwon;S. Y. Hong;C. K. Park;K. H. Park
  12. US patent 0400042 Diagnostic reagent for the determination of amylase Thomas, H.;Misson Viejo CA.
  13. Microbial enzymes and biotechnology(2nd ed) Recent advances in microbial amylase Fogarty, W. M.;C. T. Kelly;W. M. Fogarty(ed.);C. T. Kelly(ed.)
  14. US patent 53042723 Biologically pure culture of maltopentaose forming amylase producing alkalophilic bacteria Schmid, G.;C. A. Bock
  15. Ph.D. Dissertation, Faculty of dental science, Chonnam National University Identification of Microbispora rosea producing mutanase and chemical properties of mutanase Chung, J.
  16. Anal. Biochem. v.195 Miniaturization of three carbohydrate analyses using a microsample plate reader Fox, J. D.;J. F. Robyt
  17. Kor. J. Appl. Microbiol. Biotechnol. v.26 Enzymatic synthesis of new oligosaccharides using glucansucrase Baek, J. S.;D. M. Kim;J. H. Lee;P. S. Chang;N. S. Han;J. F. Robyt
  18. Carbohydr. Res. v.245 Interpertation of dextransucrase inhibition at high sucrose concentration Tanirseven, A.;J. F. Robyt
  19. Ann. RCNBMA. v.4 Characteristics of dextransu-crase of Leuconostoc mesenteroides B-1299 mutants and enzymatic modification of the amylose and pullulan Kim, D. M.;J. S. Baek;J. H. Lee
  20. Bergey's manual of Systematic Bacteriology Stanley, T.;M. Williams;E. Sharpe;J. G. Holt
  21. Microbiological Applications, Complete Version, Laboratory manual in general microbiology(sixth Edition) Johnson, M.;J. E. DeShaw;J. K. Brimeyer;L. A. Schrandt;J. E. Watters
  22. Agr. Biol. Chem. v.49 Purification and properties of an amylase from Bacillus cereus NY-14 Yoshigi, N.;T. Chikano;M. Kamimura
  23. Biotechnol. Appl. Biochem. v.28 Production and properties of a raw-starch-degrading amylase from the thermophilic and alkaliphilic Bacillus sp. TS-23 Long-Liu;L. C. Chyau;W. Hsu
  24. Handbook of amylases and related enzymes: Their sources, isolation methods, Properties and application Yamamoto, T.;S. Endo;K. Mizokamin;K. Kainuma;K. Fujita;The amylase research society of Japan(eds.)
  25. J. Biol. Chem. v.267 Catalytic properties of the coloned amylase from Bacillus licheniformis Kim, I. C.;J. H. Cha;J. R. Kim;S. Y. Jang;B. C. Seo;T. K. Cheong;D. S. Lee;Y. D. Choi;K. H. Park
  26. Biochem. v.33 The active center of a mammalian α-amylase. Structure of the complex of a panceratic α-amylase with a carbohydrate inhibitor refined to 2.2A Qian, M.;R. Hase;G. Buisson;E. Duee;F. Payan