KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Isolation of a Halotolerant Yeast and the Production of Extracellular Protease

내염성 효모의 분리 및 세포외 Protease의 생산

  • 정승찬 (배재대학교 유전공학과·생물의약 연구센타) ;
  • 현광욱 (배재대학교 유전공학과·생물의약 연구센타) ;
  • 김재호 (배재대학교 유전공학과·생물의약 연구센타) ;
  • 이종수 (배재대학교 유전공학과·생물의약 연구센타)
  • Published : 2001.04.01
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Abstract

A halotolerant and extracellular protease-producing yeast was isolated from traditional Meju and identified as a strain of Hansenular polymorpha by investigating its microbiological characteristics. The optimum pH, temperature and NaCl concentration reauired for the growth of Hansenular polymorpha S-9 were found to be pH 6.0, 30$^{\circ}C$ and 0.5 M, respectively. Extracellular protease was produced maximally at 10 U ml(sup)-1 when Hansenular polymorpha S-9 was grown on the medium containing 1.0% beef extract and 0.1 M NaCl for 12 hr at 30$^{\circ}C$. About 13% of the angiotensin-converting enzyme (ACE) inhibitory activity was shown in the hydrolysates which were obtained from the digestion of soybean protein (6 mg) for 6 hr at 30$^{\circ}C$ by the crude enzyme (1 U).

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References

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