Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
권호 표지

Effect of Dietary Fatty Acid and Vitamin E Supplementation in Antioxidant Systmes of the Second Generation Rat Brain Sections

식이지방산 조성 및 비타민 E의 보충이 제 2 세대 흰 쥐 뇌조직의 항산화 체계에 미치는 영향

  • 황혜진 (연세대학교 생활과학대학 식품영양학과) ;
  • 엄영숙 (연세대학교 생활과학대학 식품영양학과) ;
  • 정은정 (강남대학교 교양학부) ;
  • 김수연 (연세대학교 생활과학대학 식품영양학과) ;
  • 이양자 (연세대학교 생활과학대학 식품영양학과)
  • Published : 2001.01.01
Download PDF
⟨ Previous Next ⟩

Abstract

In this study, we examined the effects of dietary fatty acids and vitamin E supplementation on antioxidant systems in the rat brain regions. The Sprague Dawley rats were fed the experimental diets 3-4 wks prior to the conception. Experimental diet consisted of 10% fat(wt/wt) which were safflower oil(SO, poor in $\omega$3 fatty acids), mixed oil(MO, P/M/S ratio=1.03:1.45:1,$\omega$6/$\omega$3 ratio=6.3) and mixed oil supplemented with vitamin E(ME:MO+500mg vitamin E/kg diet). At 3 and 9 weeks of age of the newborn rats, frontal cortex(FC), corpus striatum(CS), hippocampus(H) cerebellum(CB) were dissected out from the whole brain. Activities of glutathione peroxidase(GSH-P(sub)x, superoxide dismutase(SOD) concentrations of malondialdehyde(MDA) were mesaured. Dietary fatty acids were not effective in antioxidative system for rat brain. However, when vitamin E was supplemented to the diet(ME), the activities of GSH-P(suh)x tended to increase in comparison to MO group. Therefore, the activites of GSH-P(suh)x of FC and H at the age of 3 weeks showed significant differences(p<0.05). The activities of Total-SOD tended to decrease in ME group compared to MO group. There were significant differences(p<0.05) in FC and CS at the age of 3 weeks. The activities of Mn-SOD tended to increase and Cu, Zn-SOD tended to decrease when vitamin E was supplemented. The activity levels of antioxidative enzymes at the age of 3 weeks and 9 weeks were similar. This suggested that the activity level of antioxidative enzymes reached to the adult level at the age of 3 weeks which is the end point of lactation period. The concentrations of MDA were not altered by experimental diets. When the activities of antioxidant enzymes were compared, the activities of antioxidant enzymes were the lowest in H and FC. In conclusion, the antioxidative system were not altered by dietary fatty acid at the age of 3 weeks and 9 weeks, but the supplementation of vitamin E altered the antioxidative systems. Therefore, these findings should be considered comprehensively in scope of the balance of various antioxidative systems and their interactions(Korean J Nutrition 34(1):14-22, 2001)

Keywords

References

  1. J Nutr v.119 Effect of dietary lipids and vitamin E on in vitro lipid peroxidation in rat liver and kidney homogenates Hu M-L;Frakel EN;Leibovitz BE;Tappel AL
  2. Ann Neurol v.32 Oxygen radicals as key mediators in neurological disease : fact or fiction? Halliwell BH
  3. Curr Opin Neurol v.7 Metals and free radicals in neurodegeneration Olanow CW;Arendash GW
  4. Free Radic Biol Med v.11 Vitamin E and oxidative stress Chow CK
  5. Physiol Rev v.74 Cellular defenses against damage from reactive oxygen species Yu BP
  6. Eur J Nutr v.38 no.4 Addition of vitamin E to long-chain polyunsaturated fatty acid-enriched diets protecs neonatal tissue against peroxidation in rats Suarez A;Ramirez-tortosa M;Gil A;Faus MJ
  7. Am J Clin Nutr v.29 Tocopherols and polyunsaturated fatty acids in human tissues Breri JG;Everts RR
  8. Vitamin E : Biological activity and health benefits : Overview in vitamin E in health and disease Packer L;Packer L(ed.);Fuchs J(ed.);Dekker M(ed.)
  9. Ann NY Acad Sci v.393 The effect of vitamin E on arachidonic acid metabolism Panganamala RV;Cornwell DG
  10. Neuron v.14 Superoxide dismutase delays neuronal apoptosis : a role for reactive oxygen species in programmed cell death Greenlund LJS;Deckwerth TL;Johnson EM
  11. Biochem Biophys Res Commun v.256 Cloned prokaryotic iron superoxide dismutase protects yeast cells against oxidative stress depending on mitochondrial location Balzan R;Agius DR;Bannister EH
  12. Acta Neuro v.139 no.5 The time course and reginal variations of lipid peroxidation after diffuse brain injury in rats Hsigng JN;Wang JY;Ip SM;Ng HK;Stadlin A;Yu AL;Poon WS
  13. Acta Neurol Scand v.126 Oxidants and the central nervous system, some fundamental questions Halliwell B
  14. J Neurochem v.52 Lipid hydroperoxides inhibit reacylation of phospholipids in neuronal membranes Zaleska MH;Wilson DF
  15. J Nutr v.115 Effect of dietary polyunsaturated/saturated fatty acid ratio and dietary vitamin E on lipid peroxidation in the rat Buckingham KW
  16. European J Biochem v.47 Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase Marklund S;Marklund G
  17. J Nutr v.113 Superoxide dismutase activity and lipid peroxidation in the rat : developmental correlations affected by manganese deficiency Sheri ZC;Keen CL;Hurley LS
  18. J Lab & Clin Med v.70 no.1 Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidation Paglia DE;Valentin WN
  19. J Nutr v.117 Effects of dietary selenite, selenocysteine and selenomethionine o-selenocysteine lyase and glutathione peroxidase activities and on selenium levels in rat tissues Deagen JT;Butler JA;Beilstein MA;Whagner PD
  20. Ann NY Acad Aci v.676 Lipid peroxides as the initiating factor of atherosclerosis Naito C;Kawamura M;Yamamoto Y
  21. Gastroenterol v.107 no.1 Omega 3-lipid peroxides injure CaCo-2 cells : relationship to the development of reduced glutathione antioxidant systems Cepinskas G;Kvietys PR;Aw TY
  22. Int J Vitamin Nutr Res v.64 Increased susceptibility of liver to lipid peroxidation after ingestion of a high fish oil diet Saito M;Nakatsugawa K
  23. Physiol Rev v.59 Hydroperoxide metabolism in mammalian organs Chance B;Sies H;Boveris A
  24. J Neurochem v.64 Effect of peroxynitrite on the mitochondrial respiratory chain : Differential susceptibility and astrocytes in primary culture Bolanos JP;Heales SJR
  25. Arch Biochem biophys v.308 Inhibition of mitochondrial electron transport by peroxynitrie Radi R;Rodriguez M;Castro L;Tellerei R
  26. Neurosci Letters v.244 Influence of docosahexaenoic acid on cerebral lipid peroxide in aged rats with and without hypercholesterolemia Hossain MS;Hashimoto M;Gamoh SM
  27. J Neurochem v.72 no.3 Antioxidative Effects of docosahexaenoic acid in the cerebrum versus cerebellum and brainstem of aged hypercholesterolemic rats Hossain MS;Hashimoto M;Gamoh SM
  28. Fed Proc v.41 Mitochondrial structure and function in manganase-deficient mice Zidenberg CS;Keen CL;Lonnerdal B;Hurley LS
  29. Free radical-induced lipoperoxidation and aging, CRC Handbook of Free Radicals and Antioxidants in Biomedicine Ⅱ Lippman RD
  30. Free Rad Biol & Medi v.28 no.3 Antioxidant and oxidative status in tissues of manganese superoxide dismutase transgenic mice Ibrahim W;Lee US;Yen HC;Clair DK;Chow CK
  31. Korean J Nutr v.31 no.5 Effects of P/S rations of dietary lipids and antioxidant vitamin supplements on the level of serum lipids and liver lipid peroxidation in rats treated with DMBA Lee BJ;Park JN;Lee SS
  32. Am J Clin Nutr v.41 Changes in hepatic superoxide dismutase activity in alcoholic monkey Keen CL;Amura T;Lonnerdal B;Hurlry LS;Halsted CH
  33. Biochem & Molecular Biol Internal v.35 no.6 Cu/Zn superoxide dismutase and glutathione peroxidase during aging de Hann JB;Cristiano F;Lannello RC Kola I
  34. Am J Clin Nutr v.71 no.suppl Oxidative sturess and Alzheimer disease Christen Y
  35. Am J Clin Nutr v.71 no.suppl Vitamin E and Alzheimer disease : the basis for additional clinical trials Grundman M
  36. Brain Res v.693 Chronic antioxidant treatment improves the cognitive performance of aged rats Socci DJ;Crandall BM;Arendash GW
  37. Behav Brain Res v.51 Learning begavior in chronic vitamin E-deficient and supplemented rats : radial arm maze learning and possible avoidance response Wortwin G;Okaichi H;Woshikawa T;Ibata Y
  38. Brain Res v.510 Protective effect of alpha-tocopherol on ischemic neuronal damage in the gerbil hippocampus Hara H;Kato H;Kogure K
  39. Lipids v.26 no.1 Vitamin E and susceptibility of erythrocytes and reconstituted liposomes to oxidative stress in aged diabetics Uranos S;Midori HH;tochihi N;Matsuo M;Shiraki M;Ito H
  40. Eur J Biochem v.245 Oxidative injury of synapse and alteration by vitamin E Urano S;Asai Y;Makabe S;Matsuo M;Izumiyama N;Ohtsubo K;Endo T
  41. Am J Clin Nutr v.54 Malondialdehyde and n-3 polyunsaturated fatty acids Draper H
  42. Int J Devl Neuroscience v.13 no.8 Age related changes in antioxidant enzymes, superoxide dismutase, catalase, glutathione peroxidase and glutathione in different regions of mouse brain Hussain S;Slikker JRW;Ali SF
  43. Ann Nutr Metab v.39 no.5 Alpha-tocophero and phospholipase A2 in liver and brain of chicks posthaching : the influence of dietary fat and vitamin E Fuhrmann H;Sallmann HP
  44. Effect of detary fatty acid and vitamin E supplementation on fatty acid composition, antioxidant systems of the phopholipid in the rat brain and behavioral dvelopment Hwang HJ
  45. Age-related alterations in antioxidative defense. In Free Radicals in Aging Matsuo M
  46. Free Rad Biol Med v.12 NGF restores decrease in catalase activity and increase superoxide dismutase and glutathione peroxidase activity in the brain of aged rats Nistice G;Ciriolo MR;Fiskin K;Rotilio G
  47. Ontogenez v.27 no.3 The tissue antioxidant system in the early postnatal development of rats L'vova SP;Abaeva EM
  48. Brain Res Dev Brain Res v.66 no.1 Development of antioxidant enzymes in rat brain and in reaggregation culture of fetal brain cells Aspberg A;Tottmar O
  49. Korean J Lipid v.6 no.1 Relationships between dietary ω6/ω3 fatty acid composition, vitamin E, minerals and antioxidant enzymes Lee SK;Chung EJ;Kim SY;Lee JH;Lee-Kim YC