Effect of Ethanol Extract of Quercus mongolica Leaf as Natural Food Preservative

신갈나무 잎 에탄올 추출물의 식품보존제 효과

  • Published : 2001.04.01

Abstract

This study was investigated to determine the antimicrobial effect of the ethanol extract of Quercus mongolica leaf on microbial growth. The ethanol extract at the concentration of $250\;\mu\textrm{g}/mL\;and\;500\;\mu\textrm{g}/mL$ inhibited the growth of gram positive and gram negative food-borne disease bacteria for 40 hours in tryptic soy broth, respectively. Antimicrobial activity of the ethanol extract from Quercus mongolica leaf was not affected by pH and heat treatment. The comparision between ethanol extract and commercially available preservatives on antimicrobial activity in food system was conducted. When the 0.1% ethanol extract of Quercus mongolica leaf was added to pine needle drink and carrot juice, antimicrobial activity was similar to those of containing 0.05% benzoic acid and 0.5% grapefruit seed extract. Also addition of 2~3% ethanol extract to the soybean paste inhibited the microbial growth up to 7 week, comparable to the inhibition of 2% ethanol. Thus, this results indicate that the ethanol extract of Quercus mongolica leaf may be useful as natural antimicrobial agents.

본 연구는 독성과 안정성의 문제가 없는 천연식품 보존제의 개발을 위하여 신걸나무 잎 추출물을 이용하여 천연보존제로서의 특성 및 간으성을 조사하였다. 신갈나무 잎 에탄올 추출물은 그람양성균에 대해서 $250\;\mu\textrm{g}/mL$, 그람음성균에 대해서는 $500\;\mu\textrm{g}/mL$ 농도에서 40시간 동안 식중독균의 증식을 억제하였다. 신갈나무 잎 추출물의 항균활성 물질은 pH와 열에 매우 안정하였다. 신갈나무 잎 에탄올 추출물과 합성보존제가 솔잎 음료, 당근 쥬스, 된장 및 막걸리의 보존성에 미치는 영향을 비교한 결과, 솔잎 음료 및 당근 쥬스의 경우 0.1% 첨가시 합성보존제와 유사한 미생물 생육억제 효과를 나타내었고, 된장은 추출물을 2~3% 첨가하였을 경우 7주까지는 미생물 중식이 억제되어 에틸알코올과 비슷한 보존효과를 보였다. 막걸리의 경우는 추출물 0.5% 이상 첨가시 합성보존제인 안식향산과 유사한 미생물 생육 억제효과를 나타내었다. 따라서 이와같은 결과로 미루어 보아 신갈나무 잎은 향후 천연보존제의 원료로서 사용할 수 있을 것으로 기대된다.

Keywords

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