트립토판 중합요소 알파 소단위체 $Pr28$longrightarrowLeu 잔기 치환체의 구조 변화

  • 김은주 (부산대학교 자연과학대학 분자생물학과) ;
  • 신혜자 (동서대학교 응용공학부 환경공학전공) ;
  • 임운기 (부산대학교 자연과학대학 분자생물학과)
  • 발행 : 2001.02.01

초록

A mutant tryptophan synthase $\alpha$-subunit, where Pro28 was replaced with Leu, tends to be expressed in recombinant E. coli. CD and fluorescence spectra of this protein indicate some changes in secondary and tertiary structure. Wild type protein was more or less affected by {TEX}$Ca^{2+}${/TEX} ion in regards of the fluorescent properties of its native, unfolded and intermediate forms, but the mutant protein was not at all. The dramatic structural changes may be related to the aggregation of this mutant protein.

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