Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
권호 표지
DOI QR코드

DOI QR Code

Frit-Inlet Asymmetrical Flow Field-Flow Fractionation (FI-ARIFF): A Stopless Separation Technique for Macromlecules and Nanopariticles

  • Published : 20010400
⟨ Previous Next ⟩

Abstract

This article gives an overview of a recently developed channel system, frit-inlet asymmetrical flow field-flow fractionation (FI-AFlFFF), which can be applied for the separation of nanoparticles, proteins, and water soluble polymers. A conventiona l asymmetrical flow FFF channel has been modified into a frit-inlet asymmetrical type by introducing a small inlet frit near the injection point and the system operation of the FI-AFlFFF channel can be made with a great convenience. Since sample components injected into the FI-AFlFFF channel are hydrodynamically relaxed, sample injection and separation processes proceed without interruption of the migration flow. Therefore in FI-AFlFFF, there is no requirement for a valve operation to switch the direction of the migration flow that is normally achieved during the focusing/relaxation process in a conventional asymmetrical channel. In this report, principles of the hydrodynamic relaxation in FI-AFlFFF channel are described with equations to predict the retention time and to calculate the complicated flow variations in the developed channel. The retention and resolving power of FI-AFlFFF system are demonstrated with standard nanospheres and protreins. An attempt to elucidate the capability of FI-AFlFFF system for the separation and size characterization of nanoparticles is made with a fumed silica particle sample. In FI-AFlFFF, field programming can be easily applied to improve separation speed and resolution for a highly retaining component (very large MW) by using flow circulation method. Programmed FI-AFlFFF separations are demonstrated with polystyrene sulfonate standards and pululans and the dynamic separation range of molecular weight is successfully expanded.

Keywords

References

  1. John Wiley & Sons, Inc., N.Y. Field-Flow Fractionation Handbook Schimpt, M.(ed.);Caldwell, K. D.(ed.);Giddings, J. C.(ed.)
  2. Science v.260 Giddings, J. C.
  3. Sep. Sci v.1 Giddings, J. C.
  4. Anal. Chem. v.42 Hovingh, M. E.;Thompson, G. E.;Giddings, J. C.
  5. Anal. Chem. v.46 Yang, F. J. F.;Myers, M. N.;Giddings, J. C.
  6. ACS Symposium Ser. 521 In Chromato-traphy of Polyers: Characterization by SEC and FFF Ratanathanawongs, S. K.;Giddings, J. C.;Provder, T.(ed.)
  7. Anal. Chem. v.68 Lee, S.;Rao, S. P.;Moon, M. J.;Giddings, J. C.
  8. Anal. Chem. v.64 Benincasa, M. A.;Giddings, J. C.
  9. Anal. Chem. v.64 Ratanathanawongs, S. K.;Giddings, J. C.
  10. J. Chromatogr v.813 no.1 Moon, M. H.;Kim, Y. H.;Park, I.
  11. Anal. Chem. v.59 Wahlund, K. G.;Giddings, J. C.
  12. J. Chromatogr v.461 Wahlund, K. G.;Litwen, A.
  13. Anal. Chem. v.62 Litwen, A.;Wahlund, K. G.
  14. Anal. Chem. v.61 Lee, S.;Myers, M. N.;Gidding, J. C.
  15. Anal. Chem. v.62 Giddings, J. C.
  16. Anal. Chem. v.63 Liu, M. K.;Williams, P. S.;Myers, M. N.;Gidding, J. C.
  17. Anal. Chem. v.69 Moon, M. H.;Kwon, H. S.;Park, I
  18. J. Loq.Chrom & Rel. Technol. v.20 Moon, M. H.;Kwon, H. S.;Park, I
  19. Anal. Chem. v.71 Moon, M. H.;Williams, P. S.;Kwon, H. S.
  20. Submitted Moon, M. H.;Williams, P. S.;Kang, D.
  21. Anal. Chem. v.48 Gidding, J. C.;Yang, F. J. F.;Myers, M. N.
  22. Anal. Chem. v.71 Lee, W. J.;Min, B. R.;Moon, M. H.
  23. J. Microcolumn Sep. v.11 Moon, M. H.;Lee, W. J.;Min, B. R.
  24. Anal. Chem. v.59 Williams, P. S.;Giddings, J. C.
  25. Anal. Chem. v.52 Kirland, J. J.;Yau, W. W.;Doerner, W. A.;Grant, J. W.
  26. J. Microcolumn Sep. v.9 Moon, M. H.;Lee, W. J.;Min, B. R.
  27. Part. Sci. Tecjnol. v.12 Bectett, R.;Jiang, Y,;Moon, M. H.;Liu, G.;Giddings, J.
  28. ACS Symposium Series 521, Americal Chemical Society In chromatography of Polymers Myers, M. N.;Chen, p0.;Gidding, J. C.;Provder, T.(ed.)
  29. Anal. Chem. v.53 Kirkland, J. J.;Rementer, S. W.;Yau, W. W.
  30. Sep. Sci.Technol v.16 yau, W. W.;Kirkland, J. J.
  31. Anal. Chem. v.50 Gidding, J. C.;Caldwell, K. D.;Moellmer, J. F.;Dickinson, T. H.;Myers, M. N.;Martin, M.
  32. J. Microcolumn Sep. v.9 Williams, P. S.