Journal of Acupuncture Research

Korean Acupuncture & Moxibustion Medicine Society (대한침구의학회)
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Difference in NOS between 2 Hz and 100 Hz EA in cerebral cortex, brain stem and cerebellum of spontaneously hypertensive rats

전침자극이 Spontaneously Hypertensive Rat의 대뇌겉질, 뇌줄기, 소뇌 부위의 Nitric Oxide Synthase 신경세포에 미치는 영향

  • Kim, Jong-In (Department of Acupuncture & Moxibustion, College of Oriental Medicine in Kyung-Hee University) ;
  • kim, Yong-Suk (Department of Acupuncture & Moxibustion, College of Oriental Medicine in Kyung-Hee University) ;
  • Kim, Chang-Hwan (Department of Acupuncture & Moxibustion, College of Oriental Medicine in Kyung-Hee University)
  • 김종인 (경희대학교 부속 한방병원 침구과) ;
  • 김용석 (경희대학교 부속 한방병원 침구과) ;
  • 김창환 (경희대학교 부속 한방병원 침구과)
  • Received : 2001.06.28
  • Accepted : 2001.07.21
  • Published : 2001.08.20
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Abstract

Background and Objetive : The aim of this study was to investigate the effect of various electroacupuncture stimulation on NADPH-diaphorase in cerebral cortex, brain stem, cerebellum of spontaneously hypertensive rats. Materials and Methods : We evaluated the changes of NADPH-d-positive neurons using a histochemical method. The staining intensity of NADPH-d-positive neurons was assessed in a quantitative fashion using a microdensitometrical method based on optical density by means of an image analyzer. Results and Conculsion : The average optical density of NADPH-d-positive neurons of 100 Hz (bipolar square wave 0.2 ms duration and 100 Hz frequency) electroacupuncture treatment group significantly increased in most cortical areas comparison between the manual acupuncture and 2 Hz (bipolar square wave 0.2 ms duration and 2 Hz frequency) electroacupuncture groups. In the brain stem, the optical density of NADPH-d-positive neuron at only superficial gray layer of the superior colliculus area was same as cerebral cortex. We conclude that the morphological evidence for NADPH-d-positive neurons may be have regional change in cerebral cortex brain stem and cerebellum according to various electroacupuncture stimulations.

Keywords

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