Journal of Gastric Cancer
- Volume 1 Issue 3
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- Pages.129-135
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- 2001
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- 2093-582X(pISSN)
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- 2093-5641(eISSN)
Clonality Assay of Dysplastic Epithelial Lesions of the Stomach
위 이형성 상피 병변의 클론성에 대한 분자병리학적 연구
- Choi Ho Soo (Departments of Pathology College of Medicine, Chonnam National University) ;
- Kim Mi Sook (Departments of Pathology, College of Medicine, Chonnam National University) ;
- Park Jae Woo (Departments of Pathology, College of Medicine, Chonnam National University) ;
- Park Chang Soo (Departments of Pathology, College of Medicine, Chonnam National University) ;
-
Kim Young-Jin
(Departments of Surgery, College of Medicine, Chonnam National University) ;
-
Juhng Sang-Woo
(Departments of Pathology, College of Medicine, Chonnam National University)
- 최호수 (전남대학교 의과대학 병리학교실) ;
- 김미숙 (전남대학교 의과대학 병리학교실) ;
- 박재우 (전남대학교 의과대학 병리학교실) ;
- 박창수 (전남대학교 의과대학 병리학교실) ;
-
김영진
(전남대학교 의과대학 외과학교실) ;
-
정상우
(전남대학교 의과대학 병리학교실)
- Published : 2001.09.01
Abstract
Purpose: Dysplasia or flat adenoma of the stomach is regarded as a precancerous lesion. However, the frequency and the evolutionary process of malignant transformation of gastric dysplasia are still debated. In order to see whether the lesion was a monoclonal or a polyclonal proliferation, clonality was assayed by X-linked HUMARA polymorphism. Materials and Methods: DNA was extracted from the paraffin-embedded tissue of 16 consecutive cases of endoscopic biopsy, eight of which supplied both dysplastic and nondysplastic tissue for comparison. HUMARA was amplified by PCR with or without pretreatment with methylationsensitive restriction enzyme, HpaII. The amplification products were electrophoresed on polyacrylamide gel and silver-stained. Results: Among the 16 cases, 13 cases were informative and 3 cases noninformative. Of the 13 cases, one case showed skewed lyonization, rendering 12 cases to be analyzed further. A monoclonal band pattern was noted in 2 cases, and a polyclonal band pattern in 10 cases. A review of the histopathologies of the monoclonal and the polyclonal cases did not reveal features discriminating the two groups. Conclusion: These results suggest that gastric dysplasia is a disease entity heterogeneous in the genetic level, and many cases may be non-neoplastic.