Radiation Oncology Journal
- Volume 19 Issue 4
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- Pages.381-388
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- 2001
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- 2234-1900(pISSN)
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- 2234-3156(eISSN)
Inductoin of Radioresistance by Overexpression of Glutathione S-Transferase K1 (hGSTK1) in MCF-7 Cells
MCF-7 세포주에서 Glutathione S-Transferase K1 (hGSTK1) 과발현에 의한 방사선 내성의 유도
- Kim, Jae-Chul (Department of Radiation Oncology, School of Medicine, Kyungpook National University) ;
- Shin, Sei-One (Department of Radiation Oncology, School of Medicine, Yeungnam University)
- Published : 2001.12.01
Abstract
Purpose : This study was conducted to assess the effects of x-irradiation on the expression of the novel glutathione S-transferase K1 gene. Materials and methods : Human glutathione S-transferase K1 (hGSTK1) DNA was purified and ligated to a pcDNA3.1/Myc-His(+) vector for the overexpression of hGSTK1 gene. MCF-7 cells were transfected with or without the recombinant hGSTK1 gene, and irradiated with 6 MV x-ray. After incubation of 14 days, cell survival was measured and compared. The expression of hGSTK1 and the effect of x-irradiation on hGSTK1 expression were also estimated in MCF-7 cells transfected with or without the hGSTK1 gene by RT-PCR. Results : Following 2 to 12 Gy of x-irradiation, the cell survivals were higher in the MCF-7 cells transfected with the hGSTK1 gene than in those without transfection. Despite the higher cell survival in the hGSTK1-transfected cells, RT-PCR for hGSTK1 mRNA revealed no significant differences according to radiation dose, fractionation, and time after irradiation. Conclusion : The MCF-7 cells transfected with the hGSTK1 gene showed higher cell survival than those without transfection of the gene. The hGSTK1 gene might be associated with the radiosensitivity of MCF-7 cell line and further analysis should be needed.
목적 : 사람의 유방암 세포인 MCF-7 세포주를 대상으로 gultathione S-transferase K1 (hGSTK1) 유전자의 발현 정도 및 방사선 조사에 의한 hGSTK1 유전자의 발현 변화를 관찰하고 hGSTK1 유전자를 과발현시킴으로써 hGSTK1이 방사선 감수성에 어떤 영향을 미치는 지를 관찰하였다. 재료 및 방법 : 사람의 모유두 세포 pBluescript phagemid cDNA library로부터 선별한 hGSTK1 cDNA를 pcDNA3.1/Myc-His (+) vector에 결합시킨 후, 사람의 유방암 세포인 MCF극 세포에 이입시켰다. hGSTK1 유전자를 이입시키지 않은 MCF극 세포와 hGSTK1을 이입시킨 MCF-7 세포에