Journal of Oriental Neuropsychiatry (동의신경정신과학회지)

The Korean Society of Oriental Neuropsychiatry (대한한방신경정신과학회)
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A Study on the Effects of Sunghyangjungkisan-ga-pogokyoung on In vitro Alzheimer's Disease Experimental Model

생체외(生體外) 알츠하이머병 실험(實驗) 모델에서 성향정기산가포공영(星香正氣散加蒲公英)의 효과(效果)에 관(關)한 연구(硏究)

  • Kang Hyung-Won (Dept. of Neuropsychiatry, College of Oriental Medicine, Wonkwang Univ.) ;
  • Lyu Yeoung-Su (Dept. of Neuropsychiatry, College of Oriental Medicine, Wonkwang Univ.) ;
  • Park Jin-Sung (Dept. of Neuropsychiatry, College of Oriental Medicine, Wonkwang Univ.)
  • 강형원 (원광대학교 한의과대학 신경정신과교실) ;
  • 유영수 (원광대학교 한의과대학 신경정신과교실) ;
  • 박진성 (원광대학교 한의과대학 신경정신과교실)
  • Published : 2001.12.30
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Abstract

Astrocytes are glial cells that play a major role in the inflammation observed in Alzheimer's disease (AD). Upon stimulation from various agents, these cells adopt a reactive phenotype, a morphological hallmark in AD pathology, during which they themselves may produce still more inflammatory cytokines. Substance P (SP) can stimulate secretion of tumor necrosis $factor-\;{\alpha}$ $(TNF-\;{\alpha})$ from astrocytes stimulated with lipopolysaccharide (LPS). Here I report that Sunghyangjungkisan- ga- pogokyoung(Sgp) can modulate cytokines secretion from primary cultures of rat astrocytes. Sgp $(10\;to\;1000\;{\mu}g/ml)$ significantly inhibited the $TNF-\;{\alpha}$ secretion by astrocytes stimulated with LPS and SP. Interleukin-1 (IL-1) has been shown to elevate $TNF-\;{\alpha}$ secretion from LPS-stimulated astrocytes while having no effect on astrocytes in the absence of LPS. Treatment of Sgp $(10\;to\;1000\;{\mu}g/ml)$ to astrocytes stimulated with both LPS and SP decreased IL-1 secretion significantly. The secretion of $TNF-\;{\alpha}$ by LPS and SP in astrocytes was progressively inhibited with increasing amount of IL-1 neutralizing antibody. Neurodegenerative processes in AD are thought to be driven in part by the deposition of ${\beta}\;-amyloid\;(A\;{\beta})$, a 39- to 43-amino acid peptide product resulting from an alternative cleavage of amyloid precursor protein. Sgp $(10\;to\;1000\;{\mu}g/ml)$ significantly inhibited the $TNF-\;{\alpha}$ secretion by astrocytes stimulated with $A-{\beta}-$and IL-1. These results suggest that Sgp may inhibit $TNF-\;{\alpha}$ secretion by inhibiting IL-1 secretion and that Sgp has an antiinflammatory activity in AD brain

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