Effects of Bombycis corpus on Amyloid-induced Lipid Peroxidation Antioxidative Enzymes and NO Synthesis in Rat Astrocytes

흰쥐의 뇌 astrocyte에서 $amyloid-{\beta}25-35$로 유발된 지질의 과산화와 항산화 효소계 및 NO 생성에 미치는 백강잠의 효과

  • Kim, Hee-Joon (Dept. of Internal Medicine, Oriental Medical College, Dongguk Univ.) ;
  • Jeong, Ji-Cheon (Dept. of Internal Medicine, Oriental Medical College, Dongguk Univ.) ;
  • Yoon, Cheol-Ho (Dept. of Internal Medicine, Oriental Medical College, Dongguk Univ.)
  • 김희준 (동국대학교 한의과대학 내과학교실) ;
  • 정지천 (동국대학교 한의과대학 내과학교실) ;
  • 윤철호 (동국대학교 한의과대학 내과학교실)
  • Published : 2001.09.30

Abstract

목적 : 본 연구는 식풍해경(熄風解痙), 소산풍열(疏散風熱) 효능이 있는 백강잠이 치매에 미치는 영향을 알아보기 위하여 실험을 행하였다. 방법 : 치매 유발물질인 $amyloid{\beta}(A{\beta})$ 25-35를 흰쥐의 뇌 astrocyte에 처리한 후 대표적인 항산화 효소인 catalase, superoxide dismutase,glutathione peroxidase 및 glutathione-S-transferase의 활성 변화와 NO 생성 변화를 관찰하였다. 결과 $A{\beta}$ 25-35 처리로 catalase와 superoxide dismutase 활성이 현저히 감소하였으나 백강잠을 처리한 경우는 이들 효소 활성이 크게 증가하였다. 그리고, $A{\beta}$ 25-35의 농도에 의존적으로 증가된 NO 생성은 백강잠의 농도에 의존적으로 유의성 있게 억제되는 것으로 나타났다. 결론 : 백강잠은 항산화계 효소의 활성화 및 $A{\beta}$처리와 같은 치매유발 물질의 독성에 대한 적응능력 향상을 통하여 astrocyte를 보호하는 효능을 가지는 것으로 사료되며, 아울러 노인성 치매 등 임상적 응용에 그 효과가 기대된다.

Keywords

References

  1. Prog Brain Res v.101 Neuropathological changes in Alzheimer's disease Terry, R.D.
  2. Neuroscience v.54 Molecular profile of reactive astrocytes : implications for their role in neurologic disease Eddleston, M.;Mucke, L.
  3. Human Pathol v.26 Glial cytokines in Alzheimer's disease : review and pathogenic implication Mrak, R.E.;Sheng, J.G.;Griffin, W.S.T.
  4. Neurobiol Aging v.17 Beta-amyloid converts an acute phase injury response to chronic injury responses Cotman, C.W.;Tenner, A.J.;Cummings, B.J.
  5. Neurobiol Aging v.17 Inflammatory mechanisms in neurodegeneration and Alzheimer's disease : the role of the complement system Pasinetti, G.M.
  6. J. Neurochem. v.67 Neurotoxicity of a carboxyl-terminal fragment of the Alzheimer's amyloid precursor protein Kim, S.H.;Suh, Y.H.
  7. Am. J. Pathol. v.142 Structure-activity analyses of-amyloid peptides : contributions of the 25-35 region to aggregation and neurotoxicity Pike, C.J.;Walencewicz-Wasserman, A.J.;Kosmoski, J.;Cribbs, D.H.;Glabe, C.G.;Cotman, C.W.
  8. Brain Res. v.654 Nordihydroguaiaretic acid protects hippocampal neurons against amyloid beta-peptide toxicity, attenuates free radical and calcium accumulation Goodman, Y.;Steiner, M.R.;Steiner, S.m.;Mattson, M.P.
  9. Brain Res. v.621 Calcium destablizing and neurodegenerative effects of aggregated beta-amyolid peptide are attenuated by basic FGF Mattson, M.P.;Tomaselli, K.J.;Redel, R.E.
  10. Proc. Natl. Acad. Sci. v.90 Amyloid beta-protein as a substrate interacta with extracellular matrix to promote neurite outgrowth Koo, E.H.;Park, L.;Selkoe, D.J.
  11. Brain Res. v.785 Amyloid-peptide activates cultured astrocytes : morphological alterations, cytokine induction and nitric oxide release Hu, J.;Akama, K.T.;Kraft, G.A.;Chromy, B.A.;Eldik, L.J.V.
  12. J. Free.. Radic. Biol. Med. v.12 Implication of free radical mechanism in ethanol induced cellular injury Nordmann, R.;Ribiere, C.;Rouach, H.
  13. J. Free Radic. Biol. Med. v.8 Free radicals, antioxidant enzymes and carcinogenesis Sun, Y.
  14. Lancet v.344 Free radical, antioxidant and human disease : curiosity, cause or consequence? Halliwell, B.
  15. Pharmacol. Ther. v.39 Role of vitamin A and-carotene in radiation protection ; relation to antioxidant properties Seifter, E.;Mendecki, J.;Holtzman, S.;Kanofsky, J.D.;Freidenthal, E.;Davis, L.(et al.)
  16. Biol. Trace Elem. Res. v.33 Selenium, Mechanistic aspects of anticarcino-genic action Schrauzer, G.N.
  17. J. Agric. Food Chem. v.42 Antioxidative activity of the anthocyanin pigments cyanidin 3-O-D-Glucoside and cyanidin Tsuda, T.;Wantanabe, M.;Ohshima, K.;Norinobu, S.;Choi, S.W.;Kawakishi, S.(et al.)
  18. Carcinogenesis v.10 Prevention of cytotoxicity and inhibition of intracellular communication by antioxidant catechins isolated from chinese green tea Ruch, R.J.;Cheng, S.J.;Klaunig, J.E.
  19. Chung Kuo Chung Yao Tsa Chih v.17 Inhibitory effects of the extract of Bombycis corpus on monoamine oxidase in aged mice Chen, X.;Jia, Y.;Wang, B.
  20. Clinical Appication of Oriental Medicinal Herbs Lee, S.I.
  21. Pharmacol. Rev. v.43 Nitric oxide : Physiology, pathophysiology, and pharmacology Moncada, S.;Palmer, R.M.J.;Higgs, E.A.
  22. J. Biol. Chem. v.268 Nitric oxide synthase structure and mechanism Marletta, M.A.
  23. Pharm. Rev. v.43 Nitric oxide : physiology, pathophysiology and pharmacology Moncada, S.;Palmer, R.;Higgs, E.
  24. Culturing Nerve Cells Astroglia in culture Levision, S.W.;McCarthy, K.D.;Banker, G.(ed.);Goslin, K.(ed.)
  25. Catalase. Methods of enzymatic analysis Aebi, H.;Hans Ulrich Begmeyer(ed.)
  26. Methods in Enzymology Oberley, L.W.;Spitz, P.R.;Colowick, S.P.(ed.);Kaplan, N.O.(ed.);Packer, L.(ed.)
  27. Anal. Biochem. v.42 Reevaluation of assay methods and establishment of kit for superoxide dismutase activity Oyanagui, Y.
  28. J. Lab. Clin. Med. v.70 Studies on quantitative an qualitative characterization of erythrocyte glutathione peroxidase Paglia, D.E.;Valentine, W.N.
  29. Biochem. Biophys. Res. Commun. v.71 Glutathione peroxidase activity in selenium-deficient rat liver Lawrence, R.A.;Burk, R.F.
  30. J. Biochem. v.249 Glutathione-S-transferase; the first enzymatic step mercapturic acid formation Habig, W.H.;Pabst, M.J.;Jabby, W.B.
  31. Anal. Biochem. v.150 Measurement of protein using bicichoninic acid Smith, P.K.;Krohn, R.I.;Hemanson, G.T.;Mallia, A.K.;Gartner, F.H.;Provenzano, M.D.(et al.)
  32. Arch. Biochem. Biophys. v.362 Inhibitory effects of streptozotocin, tumor necrosis factor-alpha and interleukin-1beta on glucokinase activity in pancreatic islets and gene expression of GLUT2 and glucokinase Park, C.;Park, Y.G.;Lee, Y.C.;Kim, C.H.
  33. Exp. Clin. Endocrinol v.93 Survival and B-cell function of mouse pancreatic islets maintained in culture after concomitant exposure to streptozotocin and nicotinamide Strandell, E.;Eizirik, D.L.;Sandler, S.
  34. Free Radic. Biol. Med. v.14 Interaction of vitamin C and vitamin E during free radical stress in plasma ; An ESR study Sharma, M.K.;Buettner, G.R.
  35. Chem. Res. Toxicol. v.7 The role of thiols in mitochondrial susceptibility to iron and tert-butyl hydroperoxide-mediated toxicity in cultured mouse hepatocytes Shertzer, H.G.;Bannenberg, G.L.;Zhu, H.;Liu, R.M.;Moldeus, P.
  36. Chem. Pharm. Bull. v.36 Effects of repeated administration of deer antler extract on biochemical changes related to aging in senescence-accelerated mice Wang, B.X.;Zhao, X.H.;Qi, S.B.;Kaneko, S.;Hattori, M.;Namba, T.(et al.)
  37. Chem. Pharm. Bull. v.36 Stimulating effect of deer antler extract on protein synthesis in senescence acclerated mice in vivo Wang, B.X.;Zhao, X.H.;Qi, S.B.;Yang, X.W.;Kaneko, S.;Hattori, M.(et al.)
  38. Arch. Biochem. Biophys. v.280 The antioxidants of human extracellular fluids Halliwell, B.;Gutteridge, J.M.
  39. Mol. Pharmacol. v.48 Relationship of the metabolism of vitamin C and E in cultured hepatocytes treated with tert-butyl hydroperoxide Glascott, P.A. Jr.;Gilfor, E.;Farber, J.L.
  40. Anal. Biochem. v.126 Analysis of nitrate, nitrite, and [15N]nitrate in biological fluids Green, I.C.;Wagner, D.A.;Glogowski, J.;Skipper, P.L.;Wishnok, J.S.;Tannenbaum S.T.