대한한방소아과학회지 (The Journal of Pediatrics of Korean Medicine)

  • 제15권1호
  • /
  • Pages.77-104
  • /
  • 2001
  • /
  • 1226-8038(pISSN)
  • /
  • 2287-9463(eISSN)
대한한방소아과학회 (The Association of Korean Oriental Pediatrics)
권호 표지

생혜탕(生慧湯)이 흰쥐의 학습(學習)과 기억(記憶)에 미치는 영향(影響)

Effects of Saenghyetang on Learning and Memory Performances in Mice

  • 유금룡 (천도당 한의원) ;
  • 장규태 (동국대학교 한의과대학 소아과학교실) ;
  • 김장현 (동국대학교 한의과대학 소아과학교실)
  • Yu Geum-Ryoung (cheondodang oriental clinic) ;
  • Chang Gyu-Tae (Dept. of Pediatrics, College of Oriental Medicine, Dongguk University) ;
  • Kim Jang-Hyeon (Dept. of Pediatrics, College of Oriental Medicine, Dongguk University)
  • 발행 : 2001.08.15
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The effects of the oriental herbal medicine Saenghyetang(SHT, 生慧湯), which consists of Rehmanniae Radix (熟地黃 九蒸: was made by 9th steam) 40g, Corni Fructus(山茱黃) 16g, Polygalae Radix(遠志) 8g, Zizyphi Spinosae Semen(酸棗仁) 2g, Biotae Semen(柏子仁 去油: oil ingredient was removed) 20g, Poria Cocos(茯笭) 12g, Ginseng Radix(人蔘) 12g, Acori Graminei Rhizoma(石菖蒲) 2g, Sinapis Semen(白芥子) 8g, on learning ability and memory were investigated. Hot water extract(HWE) and ethanol extract(EE) from SHT were used for the studies. Learning ability and memory are related to modifications of synaptic strength among neurons that interactive. Enhanced synaptic coincidence detection leads to improved learning ability and memory. If the NMDA receptor, a synaptic coincidence detector, acts as a graded switch for memory formations, enhanced signal detection by NMDA receptors should enhance learning ability and memory. It was shown that NR2B was increased in the forebrains of oriental medicine-administrated mice, leading to enhanced activation of NMDA receptors and facilitating synaptic potentiation in response to stimulation at 10-100 Hz. These HWE-SHT treated mice exhibited that superior ability in learning and memory when performing various behavioral tasks, showing that NR2B is enhanced by HWE-SHT treatment and also is critical in gating the age-dependent threshold for plasticity and memory formation. NMDA receptor-dependent modifications, which were mediated in part by HWE administration, of synaptic efficacy, therefore, represent a mechanism for associative learning ability and memory. Results suggest that oriental medical enhancement of NR2B contributes to increase intelligence and memory in mammals On the other hand, to examine the effects of EE-SHT on the learning ability and memory in experimental mice, EE-SHT was tested on passive and active avoidance responses. The EE-SHT ameliorated the memory retrieval deficit induced by ethanol in mice, but not other memory impairments. EE-SHT(10, 20mg/100 g, p.o.) did not affect the passive avoidance responses of normal mice in the step through and step down tests, the conditioned and unconditioned avoidance responses of normal mice in the shuttle box, lever press performance tests and the ambulatory activity of normal mice in a normal condition. However, EE-SHT at 20 mg/kg significantly decrease the spontaneous motor activity during the shuttle box test, and also to extend the sleeping time induced by pentobarbital in mice. These results suggest that SHT has an ameliorating effect on memory retrieval impairments and a weak tranquilizing action.

키워드

참고문헌

  1. 학습과 기억의 구조 v.1;2;3;4;5 박찬웅
  2. Curr.Biol v.8 Synaptic plasticity. Stevens,C.F.;Sullivan,J.
  3. Nature v.361 A synaptic model of memory:long term potentiation in the hippocampus Bliss, T.B.;Collingridge,G.L.
  4. Curr.Opin.Neurobiol. v.4 Synaptic plasticity:LTP and LTD. Bear,M.F.;Malenka,R.C.
  5. Science v.258 Molecular diversity of glutamate receptors and implications for brain function. Nakanishi, S.
  6. Annu.Rev.Neurosci. v.17 Cloned glutamate receptors. Hollmann,M.;Heinemann,S.
  7. Science v.256 Heteromeric NMDA receptors:molecular and functional distinction of subtypes. Monyer,H.
  8. Neuron v.12 Developmental and regional expression in the rat brain and functional properties of four NMDA receptors. Monyer, H.;Burnashev, N.;Laurie, D.J.;Sakmann, B.;Seeburg, P.H.
  9. Nature v.368 Changing subunit composition of heteromeric NMDA receptors during development of rat cortex. Sheng,M.;Cummings, J.;Roldan, I.A.;Yan, Y.N.;Yan, I.Y.
  10. J. Neurosci. v.18 Hippocampal synaptic plasticity in mice overexpressing an embryonic subunit of the NMDA receptor. Okabe,E.
  11. Neuron v.9 Activation and desensitization of glutamate-activated channels emdiating fast excitatory synaptic currents in the visual cortex. Hestrin, S.
  12. Curr.Opin.Neurobiol. v.4 Learning and representation in speech and language. Kuho, P.K.
  13. 辨證奇聞, 錢松
  14. 東醫生理學 金完熙(外)
  15. 臟腑辨證論治 金完熙(外)
  16. 동의학의 방법론연구 尹吉榮
  17. Neuron v.22 Variablity of neurotransmitter concentration and nonsaturation of post synaptic AMPA receptors of synapses in hippoxampal cultures and slices. Liu,G.;Choi,S.;Tsien.R.W.
  18. in Techmiques in Molecular Biology Gooderham,K.
  19. Cell v.87 The essential role of hippoampal CAI NMDA receptor-dependent synaptic plasticity in spatial memory. Tsien, J.Z.;Huerta, P.T.;Tonegawa, S.
  20. Biogenic Amines v.7 Segawa, M.;Saito, N.;Nishiyama, N.
  21. Shoyakugaku v.46 Effects of Acorus gramineus Soland on learning and memory performances in mice. Zhou,Y.;Saito,H.;Nishiyama,N.
  22. Behav. Neurosci. v.111 Impaired recognition memory in patients with lesions limited to the hippocampal formation . Reed,J.M.;Squire,R.L.
  23. Neurosci v.102 Exploratory behav. Myhrer, T.
  24. Behav. Neurosci. v.106 Ischemia-induced object-recognition defects in rats are attenuated by hippocampal ablation before or soon after ischmemia. Mumby,D.G.
  25. Behav.Neurosci. v.106 Differential contribution of amygdala and hippocampus to cued and contextual fear conditioning . Phillips,R.G.;LeDoux,J.E.
  26. Behav.Neurosci. v.106 Selective impairment of long-term but not short-term conditional fear by the N-methyl-D-asparate antagonist APV. Kim, J,J.;Fanselow, M.S.;DeCola, J.P.;Landeira-Fernandez, J.
  27. The Physiology of Learning and Memory (ed. Brower,G.H.) Davis,M.;Hitchcock,J.;Rosen.J.B.
  28. 編註醫學入門 李천
  29. 本草學 全國韓醫科大學本草學敎室(編)
  30. 申氏本草學 申佶求
  31. 中藥大辭典 江蘇新醫學院
  32. Annu.Anim.Psychol. v.21 Saito,S.;Lee,C.H.;Soella,M.
  33. Science v.238 Kucharski, D.
  34. Neuropsychobiology v.17 Hock.F.J.
  35. Int. J. Neurosci. v.38 Schaeffer,K.W.;Parson,O.A.
  36. Biochem.Cell. Biol. v.65 Acheema-Dhadli, S.;Halperin, F.A.;Sonnenberg, K.;MacMillan, V.;Haplerin,M.L.
  37. Neurosci. Lett. v.89 Blozovski, D.
  38. Drug Alcohol Depend. v.10 Crawford, A.