Journal of the Korean Association of Oral and Maxillofacial Surgeons

The Korean Association of Oral and Maxillofacial Surgeons (대한구강악안면외과학회)
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IMMUNOHISTOCHEMICAL STUDY ON THE EXPRESSION OF c-erbB-2 ONCOPROTEIN IN THE ODONTOGENIC CYSTS

치성낭에서 c-erbB-2 종양 단백의 발현에 관한 면역조직화학적 연구

  • Pyo, Sung-Woon (Div. of Oral & Maxillofacial Surgery, Dept. of Dentistry College of Medicine, The Catholic University of Korea) ;
  • Kim, Chang-Hyen (Div. of Oral & Maxillofacial Surgery, Dept. of Dentistry College of Medicine, The Catholic University of Korea) ;
  • Lee, Won (Div. of Oral & Maxillofacial Surgery, Dept. of Dentistry College of Medicine, The Catholic University of Korea) ;
  • Kim, Young-Sil (Div. of Oral & Maxillofacial Surgery, Dept. of Pathology, The Catholic University of Korea)
  • 표성운 (가톨릭대학교 의과대학 치과학교실 구강악안면외과) ;
  • 김창현 (가톨릭대학교 의과대학 치과학교실 구강악안면외과) ;
  • 이원 (가톨릭대학교 의과대학 치과학교실 구강악안면외과) ;
  • 김영실 (가톨릭대학교 의과대학 병리학교실)
  • Published : 2001.02.28
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Abstract

To investigate epithelial cell proliferation reactivity in the odontogenic cysts, the expression of c-erbB-2 oncoprotein by epithelial lining was studied in odontogenic keratocyst(OKC, n=10), dentigerous cyst(DC, n=12), radicular cyst(RC, n=12) and normal dental follicle(n=7). The c-erbB-2 immunoreactivity was studied using a streptavidine- biotin- peroxidase method with polyclonal rabbit antihuman antibody to c-erbB-2 oncoprotein which is known to react with formalin fixed, paraffin-embedded sections and the intensity of staining was determined by manually. In all of 10(100%) OKCs, showed positive expression for c-erbB-2 oncoprotein compared with 10/12(83.3%) in DCs, 11/12(91.7%) in RCs and 5/7(71.4%) in normal dental follicles. The expression within OKC was higher than that of DC, RC and dental follicle but statistically not significant(p>0.05) and but may reflects underlying genetic defect. These results demonstrate differences in c-erbB-2 expression between the epithelial linings of the three major odontogenic cyst types, indicating differences in proliferation activity and differentiation processes within these lesions. And, in particular, these results are able to explain the peculiar aggressive growth pattern of OKC.

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