Isolation and Quantitative Determination of Dioscin from Smilacis Chinae Radix

토복령(土茯令, Smilacis Chinae Radix)으로부터 Dioscin의 분리 및 함량분석

  • Seo, Jung-Hak (Department of Food and Nutrition, Andong National University) ;
  • Lee, Joo-Mi (Department of Food and Nutrition, Andong National University) ;
  • Kwon, Soon-Jin (Department of Food and Nutrition, Andong National University) ;
  • Chang, Seung-Yeup (Korea Food and Drug Administration) ;
  • Lee, Kyong-Soon (College of Pharmacy, Chungbuk National University) ;
  • Son, Kun-Ho (Department of Food and Nutrition, Andong National University)
  • Published : 2001.06.30

Abstract

The methods for isolation and quantitative determination of dioscin, a spirostanol triglycoside, from the roots of Smilax china have been developed. Isolation of dioscin was achieved by silica gel and RP-18 chromatography. The HPLC method used for quantitative determination of dioscin enabled the standardization of the crude drug. It suggests that the content of dioscin in Smilax china should be above 0.01%.

Keywords