BMB Reports
- 제33권5호
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- Pages.359-365
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- 2000
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- 1976-670X(eISSN)
MAXIZYMEs: Allosterically controllable ribozymes with biosensor functions
- Kurata, Hiroyuki (Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo) ;
- Miyagishi, Makoto (Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo) ;
- Kuwabara, Tomoko (Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo) ;
- Warashina, Masaki (National Institute for Advanced Interdisciplinary Research, AIST, MITI) ;
- Taira, Kazunari (Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo)
- 투고 : 2000.09.05
- 심사 : 2000.09.05
- 발행 : 2000.09.30
초록
Ribozymes are catalytic RNAs that can cleave RNAs at specific sites, thus they have been employed to degrade a target mRNA in vivo. Development of allosterically controllable ribozymes is of great current interest, but it remained difficult to furnish such functions to ribozymes in cultured cells or in animals. Recently, we designed allosterically controllable ribozymes termed maxizymes, which have sensor arms that recognize target mRNA sequences and, in the presence of such target sequences only, they form a cavity that can capture catalytically indispensable