BMB Reports
- 제33권1호
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- Pages.92-95
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- 2000
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- 1976-670X(eISSN)
Characterization of KI-24, a Novel Murine Monoclonal Antibody with Specific Reactivity for the Human Immunodeficiency Virus-1 p24 Protein
- Shin, Song-Yub (Peptide Engineering Research Unit, Korea Research Institute of Bioscience and Biotechnology) ;
- Park, Jung-Hyun (Peptide Engineering Research Unit, Korea Research Institute of Bioscience and Biotechnology) ;
- Lee, Myung-Kyu (Peptide Engineering Research Unit, Korea Research Institute of Bioscience and Biotechnology) ;
- Jang, So-Youn (Peptide Engineering Research Unit, Korea Research Institute of Bioscience and Biotechnology) ;
- Hahm, Kyung-Soo (Peptide Engineering Research Unit, Korea Research Institute of Bioscience and Biotechnology)
- 투고 : 1999.08.28
- 심사 : 1999.10.07
- 발행 : 2000.01.31
초록
The HIV-1 p24(202-221) sequence ETINNEEEWDRVHPV HAGP contains a B-cell epitope with the earliest immune response and the highest antibody titer against anti-mouse sera obtained by immunization with p24 antigens. A novel mouse monoclonal antibody (mAb) was generated against the immunodominant B-cell epitope of the HIV-1 p24 capsid protein, p24(202-221). BALB/c mice were immunized with the four branched multiple antigenic peptide (MAP) containing the HIV-1p24(202-221) sequence, and antibody-secreting hybridoma were produced by fusion of mouse splenocytes with P3X63Ag8.653, mouse myeloma cells. One clone which produced the antigen-specific mAb named KI-24 (Isotype IgG1, light chain: