Clinical and Experimental Reproductive Medicine
- Volume 27 Issue 2
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- Pages.165-172
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- 2000
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- 2233-8233(pISSN)
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- 2233-8241(eISSN)
Subsequent Embryo Transfers (SET) on Day 2 and Day 5: It's Safety and Effectiveness
난자채취 2일과 5일에 연속으로 실시한 배아이식의 안전성과 효과
- Park, Kee-Sang (Department of Obstetrics and Gynecology, Kyungpook National University Hospital, Department of Animal Science, Teagu University) ;
- Song, Hai-Bum (Department of Animal Science, Teagu University) ;
- Lee, Taek-Hoo (Department of Obstetrics and Gynecology, Kyungpook National University Hospital) ;
- Jeon, Sang-Sik (Department of Obstetrics and Gynecology, Kyungpook National University Hospital)
- 박기상 (경북대학교병원 산부인과학교실, 대구대학교 축산학과) ;
- 송해범 (대구대학교 축산학과) ;
- 이택후 (경북대학교병원 산부인과학교실) ;
- 전상식 (경북대학교병원 산부인과학교실)
- Published : 2000.06.30
Abstract
Objective: In vitro fertilization (IVF) and a prolonging the time of culture may be helpful in establishing a viable pregnancy through a selection effect. Some embryos do not develop beyond the 4-cell stage and some may not develop to the blastocyst stage. We have evaluated the safety of SET and the outcomes of pregnancy. Methods: Sperms were treated with Ham's F-10 supplemented with 10% human follicular fluid (hFF). oocytes or fertilized oocytes were cultured in Dulbecco's Modified Eagle Medium (DMEM) with 10% or 20% hFF respectively. Up to five oocytes were inseminated with approximately 200,000 sperm cells/2 ml in each well. Fertilization was examined in the following morning and fertilized oocytes were co-cultured until embryo transfer. Vero cells for co-culture were prepared in Tissue Culture Medium - 199 (TCM-199) with 10% fetal bovine serum. At the two to four cell and blastocyst on day 2 and day 5, embryo and blstocyst grading were evaluated. Pregnancy rate was determined after transfer of human embryos at the two to four cell stage on day 2 (Group I) or subsequent transfer of embryos on day 2 and at the blastocyst stage on day 5 (Group II). For statistical analysis, Student's t-test and Chi-square (