Clinical and Experimental Reproductive Medicine
- Volume 27 Issue 4
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- Pages.405-415
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- 2000
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- 2233-8233(pISSN)
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- 2233-8241(eISSN)
Identification of a Human Y Chromosome Specific DNA Probe and Their Clinical Application by Fluorescence in situ Hybridization Techniques
사람 Y 염색체 특이 DNA Probe의 개발과 이를 이용한 FISH 기술의 임상적 적용
- Ryu, Ran-Suk (Department of Animal Science & Biotechnology, Chinju National University, SaeWha Obstetrics and Gynecology) ;
- Lee, Sang-Chan (SaeWha Obstetrics and Gynecology) ;
- Lee, Chae-Sik (SaeWha Obstetrics and Gynecology) ;
- Kim, Jong-Heung (SaeWha Obstetrics and Gynecology) ;
- Ryu, Eun-Koung (Department of Animal Science & Biotechnology, Chinju National University) ;
- Sohn, Sea-Hwan (Department of Animal Science & Biotechnology, Chinju National University)
- 류란숙 (진주산업대학교 동물생명과학과, 세화산부인과 불임/유전연구소) ;
- 이상찬 (세화산부인과 불임/유전연구소) ;
- 이채식 (세화산부인과 불임/유전연구소) ;
- 김종흥 (세화산부인과 불임/유전연구소) ;
- 류은경 (진주산업대학교 동물생명과학과) ;
- 손시환 (진주산업대학교 동물생명과학과)
- Published : 2000.12.30
Abstract
Objective: The present study was undertaken to synthesize a human Y chromosome specific probe and to confirm the usefulness of the probe for fluorescence in situ hybridization (FISH) in various types of human cells. Methods: An approximately 400 bp DNA fragment of the DYZ1 sequences was synthesized by PCR using digoxigenin labeled dUTP (dig-PCR). The fidelity of probe was tested by FISH for cultured and uncultured human lymphocytes, amniocytes, chorionic villus cells, embryos, sperms, and germ cells of seminiferous tubule. Results: The human Y chromosome specific probe hybridized specifically to Y chromosome of the cells that had been tested. This probe assigned to the Yq12 region where the DYZ1 repetitive sequence is concentrated. Conclusion: We have identified a human Y chromosome specific probe that hybridized specifically to the Y chromosome by FISH for various types of uncultured as well as cultured cells. Therefore FISH technique using human Y chromosome specific probe should be useful for clinical application as a diagnostic tool for the detection of human Y chromosome.