Clinical and Experimental Reproductive Medicine

The Korean Society for Reproductive Medicine (대한생식의학회)
권호 표지

Development of Mouse Preimplantation Embryos in Solubilized Matrigel Media

용해된 Matrigel 첨가 배지에서 착상전 생쥐 배아의 발생

  • Chung, Byung-Mok (Department of Biology, College of Natural Sciences, Kyonggi University) ;
  • Choo, Hyung-Sik (Faculty of Liberal Arts, Kyonggi University) ;
  • Kang, Byung-Moon (Department of Obstetrics and Gynecology, College of Medicine, Ulsan University) ;
  • Gye, Myung-Chan (Department of Biology, College of Natural Sciences, Kyonggi University)
  • 정병목 (경기대학교 자연과학부 생물학과) ;
  • 추형식 (경기대학교 인문예술총괄학부) ;
  • 강병문 (울산대학교 의과대학 산부인과학교실) ;
  • 계명찬 (경기대학교 자연과학부 생물학과)
  • Published : 2000.12.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objective: To verify the effect of two forms (growth factor and growthfactor-reduced) of solubilized Matrigel on the development in mouse preimplantation embryos. Methods: Late 2-cell stage eggs were cultured through the blastocyst stage in the presence of GF- or GFR-Matrigel (0.5%, v/v). Morphological development, cell number and % apoptotic nuclei of blastocyst were measured by Roecst staining and TUNEL of nuclei. Results: Morphological development, number of cells per embryo was significantly increased in the presence of GF- or GFR-Matrigel. Culture of the embryos in the GF-Matrigel gave the best result. Conclusion: Low concentration of solubilized Matrigel improved development of mouse embryos regardless of growth factor content of the Matrigel. Growth factors and extracellular matrix protein included in the Matrigel synergistically potentiated the development of mouse embryos.

Keywords