Clinical and Experimental Reproductive Medicine
- Volume 27 Issue 4
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- Pages.381-385
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- 2000
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- 2233-8233(pISSN)
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- 2233-8241(eISSN)
Development of Mouse Preimplantation Embryos in Solubilized Matrigel Media
용해된 Matrigel 첨가 배지에서 착상전 생쥐 배아의 발생
- Chung, Byung-Mok (Department of Biology, College of Natural Sciences, Kyonggi University) ;
- Choo, Hyung-Sik (Faculty of Liberal Arts, Kyonggi University) ;
- Kang, Byung-Moon (Department of Obstetrics and Gynecology, College of Medicine, Ulsan University) ;
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Gye, Myung-Chan
(Department of Biology, College of Natural Sciences, Kyonggi University)
- Published : 2000.12.30
Abstract
Objective: To verify the effect of two forms (growth factor and growthfactor-reduced) of solubilized Matrigel on the development in mouse preimplantation embryos. Methods: Late 2-cell stage eggs were cultured through the blastocyst stage in the presence of GF- or GFR-Matrigel (0.5%, v/v). Morphological development, cell number and % apoptotic nuclei of blastocyst were measured by Roecst staining and TUNEL of nuclei. Results: Morphological development, number of cells per embryo was significantly increased in the presence of GF- or GFR-Matrigel. Culture of the embryos in the GF-Matrigel gave the best result. Conclusion: Low concentration of solubilized Matrigel improved development of mouse embryos regardless of growth factor content of the Matrigel. Growth factors and extracellular matrix protein included in the Matrigel synergistically potentiated the development of mouse embryos.