Clinical and Experimental Reproductive Medicine

The Korean Society for Reproductive Medicine (대한생식의학회)
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Comparison between Percoll and Sil-Select Methods on the Human Spermatozoa Treatment

인간정자의 처리에 있어서 Percoll과 Sil-Select 방법의 비교

  • Moon, Shin-Yong (Department of Obstetrics and Gynecology, College of Medicine, Seoul National University, Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University) ;
  • Ryu, Buom-Yong (Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University) ;
  • Shin, Hyun-Ah (Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University) ;
  • Oh, Sun-Kyung (Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University) ;
  • Suh, Chang-Suk (Department of Obstetrics and Gynecology, College of Medicine, Seoul National University, Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University) ;
  • Kim, Seok-Hyun (Department of Obstetrics and Gynecology, College of Medicine, Seoul National University, Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University) ;
  • Choi, Young-Min (Department of Obstetrics and Gynecology, College of Medicine, Seoul National University, Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University) ;
  • Kim, Jung-Gu (Department of Obstetrics and Gynecology, College of Medicine, Seoul National University) ;
  • Choi, Kyu-Hong (Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University, Department of Obstetrics and Gynecology, Samsung Cheil Hospital, Sungkyunkwan University, School of Medicine) ;
  • Lee, Jin-Yong (Department of Obstetrics and Gynecology, College of Medicine, Seoul National University)
  • 문신용 (서울대학교 의과대학 산부인과학교실, 서울대학교 의학연구원 인구의학연구소) ;
  • 류범용 (서울대학교 의학연구원 인구의학연구소) ;
  • 신현아 (서울대학교 의학연구원 인구의학연구소) ;
  • 오선경 (서울대학교 의학연구원 인구의학연구소) ;
  • 서창석 (서울대학교 의과대학 산부인과학교실, 서울대학교 의학연구원 인구의학연구소) ;
  • 김석현 (서울대학교 의과대학 산부인과학교실 서울대학교 의학연구원 인구의학연구소) ;
  • 최영민 (서울대학교 의과대학 산부인과학교실, 서울대학교 의학연구원 인구의학연구소) ;
  • 김정구 (서울대학교 의과대학 산부인과학교실) ;
  • 최규홍 (서울대학교 의학연구원 인구의학연구소, 성균관대학교 의과대학 삼성제일병원 산부인과) ;
  • 이진용 (서울대학교 의과대학 산부인과학교실)
  • Published : 2000.12.30
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Abstract

Objective: To evaluate silane-coated silica particles (Sil-select) as an alternative to polyvinylpyrrolidone-coated particles (Percoll) for gradient separation of spermatozoa, for use in assisted reproduction. Methods: 20 normal semen based on WHO criteria were included in this study. Recovery of motile and morphologically normal spermatozoa after using two-layer Percoll and Sil-select gradient respectively was recorded. Motility, HOST (hypoosmotic swelling test) and the detection of malondialdehyde for LPO (lipid peroxidation) after 24 h of incubation at $37^{\circ}C$ in a 5% $CO_2$ incubator were compared. Results: Percoll (78.5%) and Sil-select (79.1%) showed a significant increase in the motility compared to ejaculate (60.9%) but no difference between Percoll and Sil-select. Normal sperm morphology significantly increased after Percoll (57.6%) and Sil-select (53.7%) compared to ejaculate (35.8%) but no difference between Percoll and Sil-select. No differences in the recovery of motile spermatozoa and motility, HOST and the production of malondialdehyde after 24 h incubation were found when comparing the use of Percoll and Sil-select. Conclusion: Sil-select seems to be an attractive alternative to Percoll for sperm separation in assisted reproduction.

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