Journal of Korean Neurosurgical Society
- Volume 29 Issue 6
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- Pages.725-730
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- 2000
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- 2005-3711(pISSN)
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- 1598-7876(eISSN)
E1B-19k does not Localize in Mitochondria nor Dimerize Bax even with the Staurosporine
E1B-19k의 세포내 위치와 Bax와의 Dimerization에 관한 연구
- Yoon, Soo Han (Department of Neurosurgery, Ajou University School of Medicine) ;
- Kim, Jin Young (Department of Neurosurgery, Ajou University School of Medicine) ;
- Park, Seung Woo (Department of Neurosurgery, Ajou University School of Medicine) ;
- Ahn, Young Hwan (Department of Neurosurgery, Ajou University School of Medicine) ;
- Ahn, Young Min (Department of Neurosurgery, Ajou University School of Medicine) ;
- Cho, Ki Hong (Department of Neurosurgery, Ajou University School of Medicine) ;
- Cho, Kyung Gi (Department of Neurosurgery, Ajou University School of Medicine)
- 윤수한 (아주대학교 의과대학 신경외과학교실) ;
- 김진영 (아주대학교 의과대학 신경외과학교실) ;
- 박승우 (아주대학교 의과대학 신경외과학교실) ;
- 안영환 (아주대학교 의과대학 신경외과학교실) ;
- 안영민 (아주대학교 의과대학 신경외과학교실) ;
- 조기홍 (아주대학교 의과대학 신경외과학교실) ;
- 조경기 (아주대학교 의과대학 신경외과학교실)
- Received : 1999.09.03
- Accepted : 1999.11.16
- Published : 2000.06.28
Abstract
Purpose : The subcellular localization of E1B-19k has been known cytosol or nuclear membrane by immunohistochemical staining and could dimerize with Bax to regulate cell death also known by the in-vitro immunoprecipitation. We planed to confirm this dimerization of E1B-19k with Bax in vivo in Cos-7 cells by using green fluorescent protein. Material and Method : We cloned E1B-19k and Bax into C3-EGFP. C3-EGFP-E1B-19k, C3-EGFP-Bax, and C3-EGFP-E1B-19k and pcDNA3-Bax were transfected into Cos-7 cells. We explored location of E1B-19k and Bax, and confirmed its dimerization with Bax in transfected living healthy Cos-7 cells by following green fluorescent protein of E1B-19k on the confocal microscope. Results : E1B-19k was located diffusely in cytoplasm and in nucleus but not in mitochondria. It prevented cell death from the apoptosis by staurosporine but its location was not changed. GFP-E1B-19k is not changed its intracellular location with Bax even with staurosporine. Conclusion : These results support that E1B-19k does not localize in mitochondria nor dimerize with Bax even with staurosporine. We could anticipate E1B-19k prevent cell death via the other dimerizing partner or pathways.