RAPD를 이용한 자란(Bletilla striata)의 유전적 다형성 분석

Identification of the Genetic Polymorphism of Bletilla striata Using RAPD

RAPD 방법을 이용하여 국내자생 자란과 일본자생 자란의 유전적 다형성을 연구하였다. PCR 결과 156개의 재현성을 보이는 band를 얻었으며 그 중 58개가 다형성을 보였고 98개는 단일성을 나타냈다. 유연관계 분석 결과 자란은 세가지의 그룹으로 분류되었다. 첫 번째 그룹은 국내 A(자생일반종), B(목포산 자생 반엽종), C(자생 반엽종) 그리고 일본품종 D와 E(일본자생 일반종)가 속한다. 국내 무늬종 B와 C의 유사도 0.806, 일본품종 D와 E는 0.778로 매우 높게 나타났다. 두 번째 그룹은 일본품종 G(일본자생 왜성종)만이 분류되었다. 그리고 세 번째 그룹은 일본자생 무늬종인 F와 H(일본자생 무늬종)가 포함되었다.

The genetic relationship of Bletilla striata native to Korea and Japan was investigated using random amplified polymorphic DNA (RAPD). The 156 reproducible DNA bands, consisted of 58 polymorphic and 98 monomorphic bands, were obtained by polymerase chain reaction (PCR) with selected 10 random primers. The 8 Bletilla lines have been classified into three groups according to the similarity coefficient obtained by RAPD analysis. The dendrogram showed overall correlationship between similarity coefficient of 0.48 and 0.84. The first group included A (Bletilla striata native to Korea), B (Bletilla striata variegated and native to Korea in Mokpo), C (Bletilla striata variegated and native to Korea), D and E (Bletilla striata native to Japan). In this group, it was showed that B and C had the most similar genetic relationship. The similarity coefficient between D and E was 0.77. D and E had a very close resemblance in plant height and flower color with A native to Korea, respectively. The second group included only G (dwarf Bletilla native to Japan) and had a different morphological character compared to other cultivars. The last group included F and H (dwarf and variegated Bletilla native to Japan) and they had a similarity of variegation.