Restorative Dentistry and Endodontics
- Volume 25 Issue 1
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- Pages.116-122
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- 2000
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- 2234-7658(pISSN)
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- 2234-7666(eISSN)
SEM STUDY ON THE BACTERIAL ADHESION TO THE DENTIN OF THE ROOT CANAL
미생물의 근관내 상아질 부착에 대한 주사전자현미경적 연구
- Jeong, Sang-Kyun (Dept. of Conservative Dentistry, College of Dentistry, Seoul National University) ;
- Oh, Tae-Seok (Dept. of Conservative Dentistry, College of Dentistry, Seoul National University) ;
- Bae, Kwang-Shik (Dept. of Conservative Dentistry, College of Dentistry, Seoul National University)
- Published : 2000.03.05
Abstract
On the instrumented root canal wall, amorphous, irregular smear layer can be observed with Scanning Electron Microscope(SEM). The purpose of this study was to evaluate the effect of the presence or absence of smear layer on the adhesion of Staphylococcus aureus to the dentin of the root canal. Human incisors, extracted within 7 days, with no caries, no fracture, no calcification of canal, were selected. After cutting crown portion at cemento-enamel junction, root canal preparation was done by modified crown-down technique using Profile and Gates - Glidden Drill. During canal preparation, 10ml physiologic saline solution(group1&3) or 10ml 3.5% NaOCl(group2&4) was used as irrigation solution. And 10ml physiologic saline solution(group1&3) or 10ml 0.5M EDTA(group2&4) was applicated for final flush. After vertical sectioning and ethylene oxide gas sterilization, samples(group1&2) were immersed into BHIYHM broth inoculated with Staphylococcus aureus (ATCC 31153) and incubated for 3hrs at