Biotechnology and Bioprocess Engineering:BBE

  • 제5권1호
  • /
  • Pages.57-60
  • /
  • 2000
  • /
  • 1226-8372(pISSN)
  • /
  • 1976-3816(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
권호 표지

Screening of Microorganisms Producing Esterase for the Production of $(R)-\beta-Acetylmercaptoisobutyric$ Acid from Methyl $(R,S)-\beta-Acetylmercaptoisobutyrate$

  • Gokul Boyapati (Biotechnology Research Centre, Indian Institute of Technology) ;
  • Lee Je-Hyuk (Microbial & Bioprocess Engineering Laboratory, Biotechnology Research Division, Korea Research Institute of Bioscience and Biotechnology) ;
  • Song Ki-Bang (Microbial & Bioprocess Engineering Laboratory, Biotechnology Research Division, Korea Research Institute of Bioscience and Biotechnology) ;
  • Panda T. (Biotechnology Research Centre, Indian Institute of Technology) ;
  • Rhee Sang-Ki (Microbial & Bioprocess Engineering Laboratory, Biotechnology Research Division, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim Chul-Ho (Microbial & Bioprocess Engineering Laboratory, Biotechnology Research Division, Korea Research Institute of Bioscience and Biotechnology)
  • 발행 : 2000.01.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

$(R)-\beta-acetylmercaptoisobutyric$ acid (RAM), a chiral compound, is an important intermediate for the chemical synthesis of various antihypertensive and congestive heart failure drugs. Microorganisms capable of converting $(R,S)-\beta-acetylmercaptoisobutyric$ acid ((R,S)-ester) to RAM were screened from soil microorganisms. A strain of Pseudomonas sp. 1001 screened from a soil sample was selected to be the best. Cells showed an activity of 540 U/mL from culture broth and the enzyme was thermostable up to $70^{\circ}C$. This strain could produce RAM asymmetrically from (R,S)-ester.

키워드

참고문헌

  1. Science v.196 Design of specific inhibitors of angiotensin-converting enzyme: New class of orally active antihypertensive agents. Ondetti, M. A., B. Rubin, and D. W. Cushman
  2. Japan Patent v.63 Samayama, T., H. Kinugasa, H. Nishimura, K. Takeyama, and K. Hosoki
  3. Biochem. v.16 Design of potent competitive inhibitors of angiotensin-converting enzyme. Carboxyalkanoyl and mercaptoalkanoyl amino acids. Cushman, D. W., H. S. Cheung, E. F. Sabo, and M. A. Ondetti
  4. Japan Kokai Tokyo Koho v.78 Iwao, J., M. Oya, E. Kato, and T. Watanabe
  5. Japan Kokai Tokyo Koho v.81 Ohashi, N., S. Nagata, and S. Katsube
  6. Biosci. Biotech. Biochem. v.57 Chemical racemization of methyl L-β-acetylthioisobutyrate. Akihiro, S., K. Yoshimasa, N. Ohsuga, R. Numazawa, and H. Ohnishi
  7. JAOCS v.72 Synthesis of four chiral pharmaceutical intermediates by biocatalysis. Patel, R. N., B. Amit, and J. S. Laszlo
  8. Biosci. Biotech. Biochem. v.56 Screening of microorganisms producing D-β-acetylthioisobutyric acid from methyl DL-β-acetylthioisobutyrate. Akihiro, S., H. Akihiko, K. Etsuko, O. Naoto, N. Ryozo, W. Ichiro, and O. Hisoa
  9. Agric. Biol. Chem. v.45 Asymmetric hydrolysis of (±)-α-substituted carboxylic acid esters with microorganisms. Iriuchijima, S. and A. Keiyu
  10. Biosci. Biotech. Biochem. v.57 Process conditions for production of D-β-acetylthioisobutyric acid from methyl DL-β-acetylthioisobutyrate with the cells of Pseudomonas putida MR-2068. Akihiro, S., O. Eiji, T. Hiroko, O. Naoto, N. Ryozo, M. Itsumi, H. Eiichi, and O. Hisao
  11. J. Am. Chem. Soc. v.103 Bifunctional chiral synthons via microbiological methods. 1. Optically active 2,4-dimethylglutaric acid monomethyl esters. Chen, C.-S., Y. Fujimoto, and C. J. Shi