Biotechnology and Bioprocess Engineering:BBE

  • 제5권1호
  • /
  • Pages.27-31
  • /
  • 2000
  • /
  • 1226-8372(pISSN)
  • /
  • 1976-3816(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
권호 표지

A Parametric Study on Ethanol Production from Xylose by Pichia stipitis

  • Lee Tae-Young (Department of Food Science & Technology and Research Center for New Bio-Materials in Agriculture, Seoul National University) ;
  • Kim Myoung-Dong (Department of Food Science & Technology and Research Center for New Bio-Materials in Agriculture, Seoul National University) ;
  • Kim Kyu-Yong (Department of Food Science & Technology and Research Center for New Bio-Materials in Agriculture, Seoul National University) ;
  • Park Kyungmoon (Ministry of Commerce, Industry and Energy) ;
  • Ryu Yeon-Woo (Department of Molecular Science and Technology, Ajou University) ;
  • Seo Jin-Ho (Department of Food Science & Technology and Research Center for New Bio-Materials in Agriculture, Seoul National University)
  • 발행 : 2000.01.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Characteristics of ethanol production by a xylose-fermenting yeast, Pichia stipitis Y-7124, were studied. The sugar consumption rate and specific growth rate were higher in the glucose-containing medium than in the xylose-containing medium. Specific activities of xylose reductase and xylitol dehydrogenase were higher in the medium with xylose than glucose, suggesting their induction by xylose. Maximum specific growth rate and ethanol yield were achieved at 30 g xylose/L concentration without formation of by-products such as xylitol and acetic acid whereas a maximum ethanol concentration was obtained at 130 g/L xylose. Adding a respiratory inhibitor, rotenone, increased a maximum ethanol concentration by $10\%$ compared with the control experiment. In order to evaluate the pattern of ethanol inhibition on specific growth rate, a kinetic model based on Luong's equations was applied. The relationship between ethanol concentration and specific growth rate was hyperbolic for glucose and parabolic for xylose. A maximum ethanol concentration at which cells did not grow was 33.6 g/L for glucose and 44.7 g/L for xylose.

키워드

참고문헌

  1. CRC Crit. Rev. Biotechnol. v.1 Ethanol production of pentose and hexoses from cellulose materials Enari, T. M.;M. L. Suihko
  2. Appl. Biochnol. v.28;29 Xylose fermentation. An ecomomic analysis Hinman, N. D.;J. D. Wright;W. Hoagland;C. E. Wyman
  3. Appl. Biochem. Biotechnol v.28 Thermophilic ethanol production investigation of ethanol yield and tolerance in continuous culture. Lynd, L. R.;H. J. Ahn;G. Anderson;P. Hill;D. S. Kersey;T. Klapatch
  4. Arch. Microbiol v.150 Ethanol production from xylose by Thermoanaerobacter ethanolicus in batch and continuous.s culture Lacis, L. S.;H. G. Lawford
  5. Appl. Microbiol. Biotechnol v.23 The fermentation of hexose sugars and pentose sugars by Candida shehatae and Pichia stipitis du Preez, J. C.;M. Bosch;B. A. Prior
  6. Appl. Microbiol. Biotechnol. v.25 Temperature profiles of growth and ethanol tolerance of xylose-fermenting yeasts Candida shehatae and Pichia stipitis du Preez, J. C.;M. Bosch;B. A. Prior
  7. Biotechnol. Bioeng. v.24 Conversion of D-xylose to ethanol by the yeast Pachysolen tannophilus Slinger, P. J.;R. J. Bothast;J. E. van Cauwenberge;C. P. Kurtzman
  8. Biotechnol. Bioeng. v.28 Direct evidence for a xylose metabolic pathway in Saccharomyces cerevisiae Batt, C. A.;S. Carvallo;D. D. Easson;M. Akedo;A. J. Sinskey
  9. Biotechnol. Lett. v.13 Combined alcoholic fermentation of D-xylose and D-glucose by four selected microvial strains: Process considerations in relation to ethanol tolerance Laplace, J. M.;J. P. Delgenes;R. Moletta;J. M. Navarro
  10. Biotechnol. Bioeng v.37 Growth, death, and oxygen up-take kinetics of Pichia stipitis on xylose Slininger, P. J.;L. E. Branstrator;R. J. Bothast;M. R. Okos;M. R. Ladisch
  11. Kor. J. Biotechnol. Bioeng. v.8 Determination of optimum conditions for xylose fermentation by Pichia stipitis Kweon, S. H.;Y. W. Ryu;J. H. Seo
  12. Process Biochem. v.26 Utilization of sugar cane bagasse hemi-cellulosic hydrolysate by Pichia stipitis for the production of ethanol Roberto, I. C.;L. S. Lacis;M. F. S. Barbosa;I. M. de Manchilla
  13. Biotechnol. Bioeng. v.51 Xylitol production by immobilized recombinant Saccharomyces cerevisiae in a continuous packed-bed biore-actor Roca, E.;N. Meinander;B. Hahn-Hagerdal
  14. Enzyme Microb. Technol. v.17 Ethanol tolerance and activity of plasma membrane ATPase in Pichia stipitis grown in D-xylose or on D-glucose Meyrial, V.;J. P. Delgenes;C. Romieu;R. Moletta;A. M. Gounot
  15. Appl. Environ. Microbiol. v.54 Induction of xylose reductase and xylitol dehydrogenase activities in Pachysolen tannophilus and Pichiastipitis on mixed sugars. Bicho, P. A.;P. L. Runnals;J. D. Cunningham;H. Lee
  16. concentration. Enzyme Microb. Technol. v.8 Xylose fermentation by Candida shehatae and Pichia stipitis: effect of pH, temperature and substrate concentration. du Preez;J. C.;M. Bosch;B. A. Prior
  17. Appl. Microbiol. Biotechnol. v.35 Xylose metabolism by Pichia stipitis: The effect of ethanol. Delgenes, J. P., R. Moletta, and J. M. Navarro
  18. Appl. Microbiol. Biotechnol. v.29 The effect of respiratory inhibitors on fermentative ability of P. stipitis, P. tannophilcus and S. cerevisiae under various conditions of aerobiosis. Lighthelm, M. E.;B. A. Prior;J. C. du Preez
  19. Enzyme Microb. Technol. v.11 Fermentation of lignocellulose hydrolysates with yeasts and xylose isomerase. Linden, T.;B. Hahn-Hagerdal
  20. Appl. Environ. Microbiol. v.55 31P nuclear magnetic resonance study of the effect of azide on xylose fermentation by Candida tropicalis. Lohmeier-Vogel, E.;K. Skoog, H. Vogel;B. Han-Hagerdal
  21. Biotechnol. Bioeng. v.27 Kinetics of ethanol inhibition in alcohol fermentation. Luong, J. H. T.