On the effects of the characteristics of the titanium oxide to the osteoblast cell culture

Statement of problem. Confusion about the relationship of surface characteristics of implant to osteoblast cell attachment. Purpose. This study attempted to bone cell attachment to the implant surface which was modified by heat. Material and methods. Commercially pure titanium grade 2, $4{\times}4mm$ sheet 40 pieces were treated for 10 minutes with ultrasonic cleaner with methylethyl ketone, ethanol, deionized distilled water, and half of the specimen 20 pieces were heat treated in $980^{\circ}C$ for 15 minutes. All 40 specimens were autoclaves. Total 6 dishes were prepared, 3 dishes were for control group, and the other 3 dishes were for heat treatment. In fourth day, cell account was done. Conculsion. The change of surface characterization by heat treatment could affect the cell attachment in the early stage however, the change of surface characterization would not be prolonged.