Korean Journal of Veterinary Research (대한수의학회지)
- Volume 40 Issue 3
- /
- Pages.463-470
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- 2000
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- 2466-1384(pISSN)
- /
- 2466-1392(eISSN)
Tracing study for the rabbit eye ball control nerve utilizing the PRV-Ba isolated in the Korea
한국분리산 PRV-Ba를 이용한 가토 안구지배신경의 추적 연구
- Park, Il-kwon (College of Veterinary Medicine, Chungnam National University) ;
- Kim, Moo-kang (College of Veterinary Medicine, Chungnam National University) ;
- Shin, Kwang-soon (College of Veterinary Medicine, Chungnam National University) ;
- Lee, Kyung-youl (College of Veterinary Medicine, Chungnam National University) ;
- Song, Chi-won (College of Veterinary Medicine, Chungnam National University) ;
-
Lee, Kang-iee
(College of Oriental Medicine, Taejon University)
;
-
Hyun, Byung-hwa
(Korea Research Institute of Bioscience & Biotechnology)
;
-
Chang, Kyu-tae
(Korea Research Institute of Bioscience & Biotechnology)
;
- Jeong, Young-gil (College of Medicine, Gonyang University)
- 박일권 (충남대학교 수의과대학) ;
- 김무강 (충남대학교 수의과대학) ;
- 신광순 (충남대학교 수의과대학) ;
- 이경열 (충남대학교 수의과대학) ;
- 송치원 (충남대학교 수의과대학) ;
-
이강이
(대전대학교 한의과대학)
;
-
현병화
(생명과학연구소)
;
-
장규태
(생명과학연구소)
;
- 정영길 (건양대학교 의과대학)
- Received : 2000.08.03
- Published : 2000.09.25
Abstract
Until now peudorabies virus(PRV) has been used a neurotracer, because of it's properties of retrograde & anterograde transport. But it's anterograde transfort is not perfect, so we tested the applicability of the Bartha strain of PRV(PRV-Ba) isolated from South Korea as a neurotracer in the visual system. We performed immunohistochemical study of the rabbit brain after intravitreal injection of the PRV-Ba. After given survival time(24, 48, 72, 96, 120, 144hrs), the brain was removed and processed immunohistochemical stain for PRV-Ba. The strong PRV immunoreactivity(PRV-ir) were almost oberserved contralaterally in oculomotor neurons, fro example Edinger-Westphal nucleus, trigerminal nucleus of pons and peritrigerminal zone but locus of innervating sensitive neurons. The latter were weak positive and selective. PRV-Ba immunoreactive neurons were stained strongly in nucleus compared to cytoplasm. This study suggests that PRV-Ba isolated from South Korea is also a useful neurotracer in the motor innervated system like other PRV-strain.