Journal of Applied Biological Chemistry
- 제43권1호
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- Pages.49-53
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- 2000
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- 1976-0442(pISSN)
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- 2234-7941(eISSN)
In vitro Metabolism of Pyribenzoxim
- Kim, Ki Young (School of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University) ;
- Kim, Jin (School of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University) ;
- Liu, Kwang Hyeon (School of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University) ;
- Lee, Hye-Suk (Bioanalysis Laboratory, College of Pharmacy, Wonkwang University) ;
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Kim, Jeong-Han
(School of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University)
- 발행 : 2000.03.31
초록
The in vitro metabolism of a new herbicide pyribenzoxim, {benzophenone O-[2,6-bis[(4,6-dimethoxy-2-pyrimidinyl)oxy]benzoyl]oxime} was studied using rice, barnyardgrass and rat liver microsomes. No metabolism of pyribenzoxim was observed with rice and barnyardgrass microsomes though the cvtochrome P450 was active, which was evidenced by the metabolism of cinnamic acid. With rat liver microsomes, four metabolites (M1, M2, M3, and M4) were produced while parent compound decreased. M1 and M2 were from the hydrolysis reactions and NADPH-dependent metabolites were M3 and M4 (major metabolite) which were hydroxylated by cytochrome P450. They were identified as bispyribac-sodium (M1), benzophenone oxime (M2), {benzophenone O-[2,6-bis[(5-hydroxy-4,6-dimethoxy-2-pyrimidinyl)oxy]-benzoyl]oxime}(M3), and {benzophenone O-[2[(5-hydroxy-4,6-dimethoxy-2l-pyrimidinyl)6-(4,.6dimethoxy-2-pyrimidinyl)oxy]benzoyl]oxime} (M4) through LC/MS/MS analyses. Based on the results obtained metabolic map of pyribenzoxim is proposed.