Journal of Applied Biological Chemistry
- Volume 43 Issue 2
- /
- Pages.109-111
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- 2000
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- 1976-0442(pISSN)
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- 2234-7941(eISSN)
Improved Optimization of Indirubin Production from Bioreactor Culture of Polygonum tinctorium
- Chung, Choong Sik (Institute of Genetic Engineering, Kyung Hee University) ;
-
Kim, Kyung Il
(Institute of Genetic Engineering, Kyung Hee University)
;
- Bae, Geun Won (Institute of Genetic Engineering, Kyung Hee University) ;
-
Lee, Youn Hyung
(Institute of Genetic Engineering, Kyung Hee University)
;
-
Lee, Hyong Joo
(Research Center for New Bio-Materials in Agriculture, Seoul National University)
;
- Chae, Young Am (Research Center for New Bio-Materials in Agriculture, Seoul National University) ;
-
Chung, In Sik
(Institute of Genetic Engineering, Kyung Hee University)
- Received : 2000.05.08
- Published : 2000.06.30
Abstract
Effect of the two-stage operation and cell concentration on indirubin production was investigated using bioreactor culture of Polygonum tinctorium. Two-stage culture was operated successfully for 110 days without any adverse effects on continuous indirubin production. Maximum indirubin concentration was found to be at 80 mg/bioreactor. Initial cell concentration significantly affected indirubin production. The indirubin production at 29.2% PCV was improved by 845%, compared to that at 5% PCV. For high-density bioreactor culture of P. tinctorium, a maximum production rate of 10.2 mg indirubin/L day was obtained. Indirubin recovery for bioreactor operation was also examined using XAD-2, XAD-4, XAD-7, and solid silicon. XAD-4 was 1.6-fold more effective than that for solid silicon in indirubin recovery.