Journal of Applied Biological Chemistry
- 제43권3호
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- Pages.147-151
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- 2000
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- 1976-0442(pISSN)
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- 2234-7941(eISSN)
Cloning and Characterization of the Major Extracellular Neutral Protease (NprM) from Bacillus megaterium ATCC 14945
- Kim, Hoon (Department of Agricultural Chemistry, Sunchon National University) ;
- Yang, Mi-Jeong (Department of Agricultural Chemistry, Sunchon National University) ;
- Jung, Kyung Hwa (Dodram Feed Mill Co., Ltd.) ;
- Kim, Jungho (Department of Agricultural Chemistry, Sunchon National University)
- 투고 : 2000.08.12
- 발행 : 2000.09.30
초록
A gene, nprM, from Bacillus megaterium ATCC 14945 was obtained by PCR using primers synthesized based on two nprM sequences from two different strains, and cloned into Escherichia coli. The gene nprM encoded an extracellular neutral protease, and the molecular mass of the expressed enzyme was estimated to be approximately 36kDa on a denaturating gel. The enzyme was activated by