Journal of Applied Biological Chemistry
- Volume 43 Issue 4
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- Pages.207-210
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- 2000
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- 1976-0442(pISSN)
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- 2234-7941(eISSN)
Selection and Directed Evolution of New Microbial Biocatalysts and Their Application to Organic Synthesis
- Asano, Yasuhisa (Biotechnology Research Center, Toyama Prefectural University)
- Received : 2000.09.11
- Published : 2000.12.31
Abstract
As a typical example of the screening for a microbial biocatalyst from nature, isolation of nitrilesynthesizing microorganisms, characterization of a new enzyme aldoxime dehydratase, and its function in the aldoxime-nitrile pathway are introduced. Catalytic properties of some of our enzymes were improved through a direct evolutionary approach.