Effects of Sophorae Radix Extracts on the Expression ofcell cycle regulatory porteins in Human Gingival Fibroblasts

고삼추출물이 치은섬유아세포의 세포주기 조절단백질 발현에 미치는 영향

  • Kim, Heung-Sik (Department of Periodontology, College of Pharmacy, Wonkwang University) ;
  • Kim, Hyun-A (Department of Periodontology, College of Pharmacy, Wonkwang University) ;
  • Yu, Yong-Ouk (Department of Oral Biochemistry, College of Dentistry, Wonkwang University) ;
  • Kang, Tai-Hyun (Department of Pharmacy, College of Pharmacy, Wonkwang University) ;
  • Kim, Youn-Chul (Department of Pharmacy, College of Pharmacy, Wonkwang University) ;
  • Kim, Tak (Department of Periodontology, College of Pharmacy, Wonkwang University) ;
  • Pee, Sung-Hee (Department of Periodontology, College of Pharmacy, Wonkwang University) ;
  • You, Hyung-Keun (Department of Periodontology, College of Pharmacy, Wonkwang University) ;
  • Shin, Hyung-Shik (Department of Periodontology, College of Pharmacy, Wonkwang University)
  • 김흥식 (원광대학교 치과대학 치주과학교실) ;
  • 김현아 (원광대학교 치과대학 치주과학교실) ;
  • 유용욱 (원광대학교 치과대학 구강생화학교실) ;
  • 강태현 (원광대학교 약학대학 약학과) ;
  • 김윤철 (원광대학교 약학대학 약학과) ;
  • 김탁 (원광대학교 치과대학 치주과학교실) ;
  • 피성희 (원광대학교 치과대학 치주과학교실) ;
  • 유형근 (원광대학교 치과대학 치주과학교실) ;
  • 신형식 (원광대학교 치과대학 치주과학교실)
  • Published : 2000.12.31

Abstract

Fibroblasts are major cellular components of gingiva and periodontal ligament. They regulate the healing process after surgery or injury. Recently, many natural medicines, whose advantages are less side effects and possibility of long-term use, have been studied for their capacity, their anti-bacterial and anti-inflammatory effects and regenerative potential of periodontal tissues. Sophorae radix have been traditionally used as an anti-bacterial and antiinflammatory drug in oriental medicine. The purpose of present study was to investigate the effects of Sophorae radix extract on cell cycle progression and its molecular mechanism in human gingival fibroblasts. Sophorae radix extracts($100{\mu}g/ml$) notably increased cell proliferation and cell activity in the human gingival fibroblasts as compared to non-supplemented controls. There was an increase in the S phase and a decrease in the G1 phase in $100{\mu}g/ml$ of Sophorae radix extracts group as compared to non-supplemented controls. The level of cyclin E and cdk 2 protein in test group was higher than that of control groups. But that of cyclin D, cdk 4, and cdk 6 was not distinguished from controls. The level of p53 protein in test group was lower than that of controls, whereas that of p21 was not different. The level of pRB protein in test group was higher than that of controls, whereas that of p16 was lower. These results indicate that the increase of cell proliferation by Sophorae radix extracts may be due to the increased expression of cyclin E and cdk 2, and the decreased expression of p53 and p16 in human gingival fibroblasts.

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