The Journal of the Korean Society for Microbiology (대한미생물학회지)

The Korea Society for Microbiology (대한미생물학회)
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Pulsed Field Gel Electrophoresis Profile of Erythromycin-Clindamycin Resistant Streptococcus pyogenes Isolated in Korea

국내분리 Erythromycin-Clindamycin 내성 Streptococcus pyogenes에 대한 Pulsed Field Gel Electrophoresis 양상 분석

  • Lee, Young-Hee (Laboratory of Molecular Bacteriology, Department of Microbiology National Institute of Health) ;
  • Hwang, Kyu-Jam (Laboratory of Molecular Bacteriology, Department of Microbiology National Institute of Health) ;
  • Lee, Kwang-Jun (Laboratory of Molecular Bacteriology, Department of Microbiology National Institute of Health) ;
  • Park, Kang-Soo (Laboratory of Molecular Bacteriology, Department of Microbiology National Institute of Health) ;
  • Bae, Song-Mee (Laboratory of Molecular Bacteriology, Department of Microbiology National Institute of Health) ;
  • Sung, Hwa-Young (Laboratory of Molecular Bacteriology, Department of Microbiology National Institute of Health) ;
  • Kim, Ki-Sang (Laboratory of Molecular Bacteriology, Department of Microbiology National Institute of Health) ;
  • Lee, Chong-Sam (Department of Biology, College of Science, Sungshin Women's University)
  • 이영희 (국립보건원 세균질환부 분자세균과) ;
  • 황규잠 (국립보건원 세균질환부 분자세균과) ;
  • 이광준 (국립보건원 세균질환부 분자세균과) ;
  • 박강수 (국립보건원 세균질환부 분자세균과) ;
  • 배송미 (국립보건원 세균질환부 분자세균과) ;
  • 성화영 (국립보건원 세균질환부 분자세균과) ;
  • 김기상 (국립보건원 세균질환부 분자세균과) ;
  • 이종삼 (성신여대 이과대학 생물학과)
  • Published : 2000.04.30
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Abstract

Ninety two strains of Streptococcus pyogenes were isolated from patients with pharyngitis, scarlet fever, skin infection, and invasive streptococcal infections in Seoul, Korea from January to December, 1998. All isolates were epidemiologically characterized by T protein serotype, and serum opacity factor (OF) detection to phenotypes. To analyze the genetic relationship, fifty two isolates including 32 erythromycin-clindamycin (Em-Cm) resistant strains, 20 antimicrobial susceptible strains were attempted to the pulsed-field gel electrophoresis (PFGE). T protein serotype showed 16 kinds in distribution including T12 and T4. Among the total isolates, 40 strains (43.5%) belonged to the T12 serotype and twenty strains (21.7%) to T4 serotype. On the other hand, when infection aspect of S. pyogenes isolates were analysed by T serotype distribution, T12 type was predominant for pharyngitidis which contributed to 21 strains (53%) and for skin infection isolates which contributed to 11 strains (28%), respectively. In case of T4 type, it was the most predominant pharyngitidis isolates which contributed to 8 strains (40%). In T serotype distribution of Em-Cm resistant strains, 27 strains (84%) of the thirty two showed T12 serotype. In minimum inhibitory concentration (MIC) values of Em-Cm resistance isolates, thirty two isolates showed resistant to erythromycin 27 strains (84%), had high MIC of >$128\;{\mu}g/ml$. And also to clindamycin, twenty two strains (69%) had high MIC of >$128\;{\mu}g/ml$. When OF detection of Em-Cm resistance of S. pyogenes isolates were analyzed by T serotype distribution, T12 serotype isolates revealed that all of the isolates except one strain were OF negative. In PFGE profile analysis to Em-Cm resistance isolates, of the twenty seven, Em-Cm resistance of T12 serotype isolates, 26 strains showed identical PFGE profile and all of these isolates revealed that OF negative. Eighty four percent of Em-Cm resistance S. pyogenes isolates had identical phenotype and PFGE profile. These results strongly suggested that the Em-Cm resistant S. pyogenes isolates from Seoul area showed close genetic correlation and PFGE could be available tool for molecular epidemiology.

Keywords

References

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