The Journal of the Korean Society for Microbiology (대한미생물학회지)

The Korea Society for Microbiology (대한미생물학회)
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Bacteriocin ("Vulnificin") Typing of Vibrio vulnificus

  • Ha, Tai-You (Department of Microbiology and Immunology, Chonbuk National University Medical School) ;
  • Lee, Jeong-Ho (Department of Microbiology and Immunology, Chonbuk National University Medical School) ;
  • Lee, Hern-Ku (Department of Microbiology and Immunology, Chonbuk National University Medical School) ;
  • Whang, Hee-Sung (Department of Microbiology and Immunology, Chonbuk National University Medical School) ;
  • Park, Jae-Seung (Department of Microbiology and Immunology, Chonbuk National University Medical School)
  • Published : 2000.06.30
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Abstract

Vibrio vulnificus, a halophilic vibrio is an estuarine gram-negative bacteria that is associated with severe and frequently fatal wound infections and life-threatening septicemia. Bacteriocins are defined as antibacterial substance produced by various species of bacteria which are usually active against closely related organisms. Bacteriocins have found widespread application in epidemiological studies as specific markers of bacteria. It was proposed by Ha et al. (1990. J. Korean. Soc. Microbiol. 25: 586.) to give the bacteriocins produced by V. vulnificus the name "vulnificins". In the present study, a total of 72 strains of V. vulnificus isolated from patients and oysters were subjected to screen potential producers and indicators of vulnificin, applying ultraviolet induction method. Sensitivity of several strains of Serratia marcesans, Pseudomonas aeruginosa, Shigella flexneri, Salmonella typhi and Yersinia enterocolitica to vulnificins were also examined out. All the tested strains of V. vulnificus produced vulnificins active against indicator strains with various different inhibitory patterns. The spectrum of vulnificin activity and sensitive spectrum of indicator strains were considerably broad. Interestingly, almost all strains of S. marcescens, P. aeruginosa, Salmonella sp., Shigella sp. and Y. enterocolitica tested were sensitive to 1-7 vulnificin(s). Taken together, the present study demonstrated that all of the isolates of V. vulnificus produced vulnificins and that 8 good vulnificin producers and 10 good indicators were detected. These strains can be employed efficiently for establishing vulnificin typing scheme of V. vulnificus and for the detection of bacteriocinogeny and sensitivity in V. vulnificus. Biological role of vulnificin remains to be further elucidated.

Keywords

References

  1. J Gen Microbiol v.19 Bacteriocin-like material produced by Pasteurella pestis Ben-Gurion R;Hertman I
  2. N Engl J Med v.300 Disease caused by a marine vibrio: clinical cheracteristics and epidermiology Blake PA;Merson MH;Weaver RE;Hollis DG;Heublin PC
  3. Vibrocin typing In Method in Microbiology v.12 Brandis H;Bergan, T(ed);JR Norris
  4. Infect Immun v.1 Bacteriocin typing of Vibrio cholerae Chakrabaty AN;Adhya, S;Basu J;Dastidar SG
  5. Clin Inf Dis v.15 Vibrio vulnificus infections in Taiwan: Report of 28 cases and review of clinical manifestations and treatment Chung YC;Yuan CY;Liu CY;Lan CK;Huang AH-M
  6. J Bacteriol v.98 Effect of vibriocins on member of Enterobacteriaceae Datta A;Prescott LM
  7. Vibriocin. Nature (Lond) Lethal biosynthesis of a new antibacterial principle Farkas-Himsley H;Seyfried PL
  8. Canad J Microbiol v.9 Lethal biosynthesis of a bacteriocin, vibriocin, by Vibrio comma. Ⅲ. Vibriocin production and sensitivity in relation to redox potentials and streptomycin resistance. Farkas-Himsley H;Seyfried PL
  9. J Immunol v.84 Mixed bacterial infections in relation to antibiotic activitis. Ⅳ. Shigella-Escherichia coli infections Friedman DR;Halbert SP
  10. Schweiz Z Allgen Patrol Bakterio v.9 Sur la specificite das actions anstibioticques Frederiq P
  11. Compt Rend Soc Biol (pairs) v.142 Production des substances antibiotiques par certaines souches de Shigella Fredericq P
  12. Compt Rend Soc Biol(pairs) v.142 Recherche des proprietees lysogenes et antibiotiques chez les Salmonella Fredericq P
  13. Colicins. Annu Rev Microbiol v.11 Fredericq P
  14. Compt Rend Soc Biol v.144 Distribution des souches productrices de colicine V dans selles mormals et pathologiques. Fredericq P;Jorris E
  15. Compt Rend Soc Biol(pairs) v.93 Sur un remarquable example d'antagonism entre deux souches de colibacille Gratia A
  16. Thesis Collection (Natural Sci) v.23 Comparative studies on bacteriocin induction methods and sensitivity of bacterial isolates to bacteriocins. Ha TY
  17. J Korean Soc Microbiol v.25 no.586 Production of a bacteriocin, "vulnificin" by Vibrio vulnificus Ha TY;Park YM;Park BS
  18. In Abstracts of the 2nd Japan-Korean International Symposium on Microbiology 1994 v.7 no.October Production and sensitivity to bacteriocin "Vulnificin" of Vibrio vulnificus, abstr. PP 83-84 Ha TY;Wang HS
  19. Bacteriol Rev v.39 Colicinogeny and related phenomena Hardy KG
  20. J Gen Microbiol v.16 Heriditary aberrancy in growth of same Bacillus megaterium strains Ivanovics G;Nagy E
  21. Compt Rend Acad Sci v.231 Induction de la lyse de la production de bacteriophages chez un Psedomonas pyocyanea lysogene Jacob F
  22. Ann Inst Pasteur v.84 Definition de quelques termes relatifs a la lysogenie Jacob F;Lwoff A;Siminovitch L;Wollman E
  23. J Bacteriol v.102 Mode of action of vibtiocin Jayawardene A;Farkas-Himsley H
  24. Ann Intern Med v.109 Syndromes of Vibrio vulnificus: Clinical and epidermiological features in Florida cases, 1981-1987. Klontz KC;Leib S;Schriber M;Janowski HT;Baby LM;Gunn RA
  25. Infect Immun v.3 Mode of action: Initial interaction with Vibrio comma cells Krol PM;Farkas-Himsley H
  26. Korean Cent J v.19 Production of antibiotic substances, vibriocins by Vibrio cholerae Biotype EL Tor. Lee Y-C
  27. Infet Immun v.30 Single bacteriocin typing scheme for the vibrio group of organisms Mitra S;Balganesh TS;Dastididar SG;Chakarabarty AN
  28. Ann Intern Med v.109 Vibrio vulnificus-a new monster of the deep? Morris JG
  29. J Bacteriol v.114 Extractable lipids of gran-negativge marine bacteria:Phospholipid composition Oliver JD;Colwell RR
  30. Biochem Biophys Acta v.18 A colicin produced by cells that are sensitive to it Ryan FJ;Mukai F
  31. Pediatria(Moscow) v.52 no.69 Colicinogenicity of the causative agent and accompanying intetinal microflora in the various forms of dysentery in children Tatarinova SD;Pozhalostrina LV;Sukhareva ME;Ermoliva ZV
  32. FDA Medical Bulletin v.6 no.March To prevent vibrio infections, high-risk patients should avoid eating raw molluscan shellfish US Food and Drug Administration
  33. J Bacteriol v.96 Production of and sensitivity to colicins among serologically classified strains of Escherichia coli Vosti KL
  34. Infect Immun v.64 Preclinical immynoprophylactic and immunotherapeutic efficacy of antisera to capsular polysaccharide-tetanus toxioid conjugate vaccines of Vibrio vulnificus Wright AC;Hill RT;Johnson JA;Rochman M-C;Colwell RR;Morris JG
  35. Appl Microbiol v.17 Pyocine typing of climical strains of Pseudomonas aeruginosa Zabransky RJ;Day FE