Journal of Korean Society of Forest Science (한국산림과학회지)

Korean Society of Forest Science (한국산림과학회)
권호 표지

Diversity of I-SSR Variants in the Populations of Torreya nucifera

비자나무 집단(集團)에서의 I-SSR 변이체(變異體)의 다양성(多樣性)

  • Hong, Yong-Pyo (Division of Genetics & Physiology, Dept. of Tree Breeding, Korea Forest Research Institute, Forestry Administration) ;
  • Cho, Kyung-Jin (Division of Genetics & Physiology, Dept. of Tree Breeding, Korea Forest Research Institute, Forestry Administration) ;
  • Kim, Yong-Yul (Division of Genetics & Physiology, Dept. of Tree Breeding, Korea Forest Research Institute, Forestry Administration) ;
  • Shin, Eun-Myoung (Division of Genetics & Physiology, Dept. of Tree Breeding, Korea Forest Research Institute, Forestry Administration) ;
  • Pyo, Sun-Kyung (Division of Genetics & Physiology, Dept. of Tree Breeding, Korea Forest Research Institute, Forestry Administration)
  • Received : 1999.11.09
  • Published : 2000.06.30
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Abstract

Inter-simple sequence repeat (I-SSR) markers were analyzed from diploid genomes of 95 nutmeg trees (Torreya nucifera Siev. et Zucc.) in 5 populations. A total of 62 I-SSR amplicons were observed and 7 of them were monomorphic in 95 individuals. DNA fingerprint of each tree was verified by pooling the observed I-SSR amplicons. Most of the genetic diversity was allocated within population (90.65%) and all the populations revealed similar level of I-SSR amplicon diversity within population. Degree of population differentiation (${\phi}_{ST}=9.35%$) was moderate on the basis of criteria obtained from isozyme analysis. Based on the results of the cluster analysis of UPGMA, genetic relationships among 5 populations were not coincided with the pattern of geographic distribution. Non-significant confidence interval at each node also suggests that all the nutmeg populations are genetically not much differentiated.

국내 5개 지역에서 채집한 비자나무(Torreya nucifera Siev. et Zucc.) 95개체를 대상으로 I-SSR 표지자를 분석하였다. 총 62개의 I-SSR 증폭산물(增幅産物)이 관찰되었으며, 그 중 7개의 증폭산물(增幅産物)은 분석된 95개 개체에서 단형성(單形性)이었다. 관찰된 전체 I-SSR 증폭산물(增幅産物)을 통합(統合)하여 분석한 결과 개체목에 대한 DNA지방판별(指放判別)이 가능하였다. 대부분의 유전다양성(遺傳多樣性)이 임분(林分)내의 개체목 간에 존재하는 것으로 나타났고(90.65%), 전체 5개 임분(林分)에서 유사한 수준의 유전다양성(遺傳多樣性)을 보였다. 집단간의 유전적(遺傳的) 분화(分化)정도는 심하지 않았다(${\phi}_{ST}=9.35%$). UPGMA법에 의한 유집분석(類集分析) 결과 각 집단의 유전적(遺傳的) 유연관계(類緣關係)는 임분(林分)의 지리적(地理的) 분포양상(分布樣相)과 일치(一致)하지 않았으며, 각 교점(交點)의 형성(形成)에 있어서 통계적 유의성이 없었고 따라서 전체 집단들이 유전적(遺傳的)으로 크게 분화(分化)되지 않았음을 알 수 있었다.

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