The Journal of Korean Society of Virology (대한바이러스학회지)

The Korean Society of Virology (대한바이러스학회)
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Typing and Sequence Analysis of the VP7 Gene of Rotavirus Isolated from Infants in Korea

한국영아에서 분리된 로타바이러스의 VP7 유전자형 및 염기서열 분석

  • Song, Mi-Ok (Department of Microbiology, Chung-Ang University College of Medicine) ;
  • Yun, Karyn (Department of Microbiology, Chung-Ang University College of Medicine) ;
  • Chung, Sang-In (Department of Microbiology, Chung-Ang University College of Medicine) ;
  • Choi, Chul-Soon (Department of Microbiology, Chung-Ang University College of Medicine) ;
  • Lim, In-Seok (Department of Pediatrics, Chung-Ang University College of Medicine) ;
  • Kang, Shien-Young (College of Veterinary Medicine, Chungbuk National University) ;
  • An, Chang-Nam (Central Research Institute, Korea Green Cross Corporation) ;
  • Kim, Won-Yong (Department of Microbiology, Chung-Ang University College of Medicine)
  • 송미옥 (중앙대학교 의과대학 미생물학교실) ;
  • 윤여란 (중앙대학교 의과대학 미생물학교실) ;
  • 정상인 (중앙대학교 의과대학 미생물학교실) ;
  • 최철순 (중앙대학교 의과대학 미생물학교실) ;
  • 임인석 (중앙대학교 의과대학 소아과학교실) ;
  • 강신영 (충북대학교 수의과대학 미생물학교실) ;
  • 안창남 (㈜ 녹십자 종합연구소) ;
  • 김원용 (중앙대학교 의과대학 미생물학교실)
  • Published : 2000.06.30
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Abstract

Rotaviruses are the most common cause of severe vomiting and diarrhea in children worldwide and classified as a genus in the family Reoviridae. Rotavirus has eleven segmented dsRNAs and the virion consists of three shells. Outer capsid VP7 and VP4 induce neutralizing antibodies and are classified into G types (glycoprotein VP7) and P types (protease-sensitive VP4). Characterization of VP7 gene of Korean isolates of human rotavirus was performed using multiplex PCR and nucleotide sequence analysis. After RT-PCR amplification of full length (1,062 bp) of VP7 genes, the amplified PCR products were G typed by multiplex PCR and the nucleotide sequences were compared with those of reference rotavirus from GenBank. The G type analysis revealed that 25% (2/8) belong to G1, whereas 37.5% (3/8) benong to G2 and G4, respectively. The Korean isolates within the same serotypes showed high homology of nucleotide sequences and could be discriminated from foreign isolates exception with two strains (CAU009 and CAU022). But Korean isolates CAU009 and CAU022 were close related into japanease isolates 417 (99.2%) and indian isolates (97.6%) than Korean isolatese. Our results showed that these two strains were supposed to be originated from abroad. As a results, The G typing and nucleotide sequence analysis of VP7 gene of rotavirus isolated from infants in Korea could be used for identification, serotying and determination of novel or unusual strains of rotaviruses.

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