The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
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Effects of Danchun-hwan on Oxidative Damage of Human Neural Cell

단천환이 사람신경세포의 산화적 손상에 미치는 영향

  • 한상혁 (원광대학교 한의학 전문대학원) ;
  • 김명선 (원광대학교 의과대학 미생물학교실) ;
  • 이지현 (원광대학교 한의학 전문대학원, 원광대학교 의과대학 미생물학교실) ;
  • 김도환 (원광대학교 한의학 전문대학원) ;
  • 나영훈 (원광대학교 한의학 전문대학원) ;
  • 조광호 (원광대학교 한의학 전문대학원) ;
  • 박래길 (원광대학교 한의학 전문대학원, 원광대학교 의과대학 미생물학교실) ;
  • 문병순 (원광대학교 한의학 전문대학원)
  • Published : 2000.12.01
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Abstract

Objectives : The present study was carried out to investigate the effects of Danchun-hwan(DCH) on the peroxynitrite-induced neural cell death in human neuroblastoma cell line, SH-SY5Y. Methods : The cultured cells were pretreated with DCH and exposed to 3-morpholinosydnonimine(SIN-1) that simultaneously generates NO and superoxide, thus possibly forming peroxynitrite. The cell damage was assessed by using MTT assay and crystal violet staining. Results : Exposure of the cells to SIN-1 for 24hr induced 75% apoptotic cell death, as evaluated by the occurrence of morphological nuclear changes characteristic of apoptosis using 4', 6-diamidino-2-phenylinole(DAPI). However, pretreatment of SH-SY5Y with the water extracts of DCH, inhibited the apoptotic cell death in a dose-dependent manner. DCH also inhibited SIN-1-induced apoptotic caspase 3-like protease activity in a dose-dependent manner. DCH recovered the depleted glutathione levels by SIN-1. Conclusions : Taken together, it is suggested that DCH protected human neuroblastoma cell line, SH-SY5Y, from the free radical injury mediated by peroxynitrite by a mechanism of elevating antioxidant, GSH.

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References

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