Toxicological Research
- Volume 16 Issue 2
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- Pages.101-107
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- 2000
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- 1976-8257(pISSN)
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- 2234-2753(eISSN)
Antitumor Evaluation of Cannabidiol and Its Derivatives by Colorimetric Methods
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Baek, Seung-Hwa
(Department of Natural Products, Professional Graduate School of Oriental Medicine and Wonkwang University)
;
- Shin, Ji-Hee (Department of Natural Products, Professional Graduate School of Oriental Medicine and Wonkwang University) ;
- Chung, Woo-Young (Department of Oral Anatomy, School of Dentistry, Wonkwang University) ;
- Han, Du-Seok (Department of Oral Anatomy, School of Dentistry, Wonkwang University)
- Published : 2000.06.01
Abstract
Cannabidiol derivatives (1, 2 and 3), 5-fluorouracil (4, 5-FU) and adriamycin (5, AM) were tested for their growth inhibitory effects against human tumor cell lines using two different 3-{4,5-dimeth-ylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay and sulforhodamine B protein (SRB) assay. The light microscopic study showed morphological changes of the treated cells. Disruptions in cell organelles were determined by colorimetric methods; MTT assay and STB assay. These results suggest that cannabidiol (1, CBD) retains the most growth-inhibitory activity against human tumor cell lines.
Keywords
- Cannabidiol;
- 5-Fluorouracil;
- Adriamycin;
- Growth-inhibitory activity;
- Cytotoxic effect;
- KB cell lines;
- SK-MEL-3 cell lines;
- MTT assay;
- SRB assay