ESR Signal in Different Cuts of Irradiated Chicken, Pork and Beef

  • Published : 2000.06.01

Abstract

Electron spin resonance (ESR) spectroscopy was used to detect irradiated meat containing bones (chicken, pork and beef), to investigate the effect of irradiation dose on the ESR signal intensity and to identify the stability of radicals under 9 weeks of storage. Chicken, pork and beef were irradiated with doses 0, 1, 3, 5 and 7 kGy at room temperature using a Co-60 irradiator. Bones were pieced and dried, which were placed in a quartz tube within an Electron paramagnetic resonance (EPR) spectrometer resonator cavity. The irradiated bone presented an asymmetric absorption in shape, different from that of a non-irradiated one. The signal intensity of smaller animals are lower than larger species. Variation was observed between samples of the same species depending on the calcification status of the bone. Moreover different irradiation doses produced different signal areas that make possible to estimate the absorbed dose of treated meat. The ESR signal stability after irradiation was stable in even after a 9 week storage at room temperature.

Keywords

References

  1. E. Ross,'Dosimetry, tolerance and shelf-life extension related to disinfestation of fruit and vegitables by gamma irradiation,'A. Rep. to the AEC., UH-235, 5-11(1965)
  2. J. F. Diehl, 'Potential and current applications of food irradiation' In Safety of irradiated foods, 2nd ed., Marcel Dekker Inc., New York, 291-339(1995)
  3. G. A. Schreiber, N. Helle, G. Schulzki, A. Spiegelberg, B. Linke, U. Wagner, and K. W. BOgl, 'Intercomparisons to evaluate the suitability of gaschromatographic, electron spin resonance spectrometric and thermoluminescence methods to detect irradiated foods in routine control,' Radiat. Phys. Chem., 42, 391-396(1993) https://doi.org/10.1016/0969-806X(93)90273-W
  4. A. Spiegelberg, L. Heide and K. W. B gl, 'Identification of irradiated chicken by GC/MS determination of radiation induced volatiles from lipids,' In Potential new methods of detection of irradiated food, J. J. Raffi, and J. J. Belliardo, BCR Information, EUR/13331/EU, Brussels, Luxembourg: Commission of the European Communities, 177-186(1991)
  5. D. R. Boyd, A. V. J. Crone, J. T. G. Hamilton, M. V. Hand, M. H. Stevenson and P. J. Stevenson, 'Synthesis, characterization, and potential use of 2-dodecylcyclo butanone as a marker for irradiated chicken,'J. Agric. Food Chem., 39, 789-792(1991) https://doi.org/10.1021/jf00004a035
  6. M. F. Desrosiers, 'Current status of the EPR method to detect irradiated food,' Appl. Radiat. Isot., 47, 11-12, 1621-1628(1996) https://doi.org/10.1016/S0969-8043(96)00255-2
  7. N. J. F. Dodd, J. S. Lea and A. J. Swallow, 'ESR detection of irradiated food,' Nature, 334, 387(1988) https://doi.org/10.1038/334387a0
  8. J. J. Raffi and S. M. Benzaria,' Identification of irradiated foods by electron spin resonance techniques,'J. Radiation. Sterilization, 1, 282-304(1993)
  9. J. J. Raffi, J. J. Belliardo, and J. P. Agnel, 'Application of ESR to identification of irradiated foodstuffs,'J. Chem. Phys., 91, 11, 1913-1929(1994)
  10. J. J. Raffi, and P. Stocker, 'Electron paramagnetic resonance detection of irradiated foodstuffs,' Appl. Magn. Reson., 10, 1-3, 357-373(1996)
  11. M. F. Desrosiers, W. L. McLaughlin, L. A. Sheahen, N. J. F. Dodd, J. S. Lea, J. C. Evan, C. C. Rowlands, J. J. Raffi, and J. P. L. Agnel,'Co-trial on ESR identification and estimates of gamma-ray and electron absorbed doses given to meat and bones,' Int. J. Food Sci. Technol., 25, 682-691(1990) https://doi.org/10.1111/j.1365-2621.1990.tb01129.x
  12. R. Gray, and M. H. Stevenson,'The effect of post-irradiation cooking on the ESR signal in irradiated chicken drumsticks,'Int. J. Food Sci. Technol., 24, 447-450(1989)
  13. R. Gray, and M. H. Stevenson, 'Detection of irradiated deboned turkey meat using ESR spectroscopy,' Radiat. Phys. Chem., 34, 899-902(1989) https://doi.org/10.1016/1359-0197(89)90327-5
  14. R. Gray, M. H. Stevenson, and D. J. Kilpatrick, 'The effect of irradiation dose and age of bird on the ESR signal in irradiated chicken drumsticks,' Radiat. Phys. Chem., 35, 284-287(1990) https://doi.org/10.1016/1359-0197(90)90102-N
  15. R. Gray, and M. H. Stevenson, 'Effect of dose rate and length of storage on the ESR signal strength in irradiated chicken bone,' Int. J. Food Sci. Technol., 26, 669-672(1991) https://doi.org/10.1111/j.1365-2621.1991.tb02011.x
  16. M. A. Jose, H. A. Empis, M. L. Silva and E. M. A. Nunes, 'Detection of irradiated fish using EPR of fish bone: Signal intensity and stability,' Fisheries Research, 21, 471-475(1995) https://doi.org/10.1016/0165-7836(94)00300-L
  17. M. F. Desrosiers, '${\gamma}$-Irradiated seafoods: Identification and dosimetry by Electron paramagnetic resonance spectroscopy,'J. Agric. Food Chem., 37, 96-100(1989) https://doi.org/10.1021/jf00085a022
  18. Raffi, J., 'Proposed standard method of EPR detection of irradiated fish,' Int. J. Food Sci. Technol., 27, 111-124(1992)
  19. J. J. Raffi, J. P. L. Agnel, L. A. Buscarlet and C. C. Martin, 'Electron spin resonance identification of irradiated strawberries,' J. Chem. Soc., Faraday Trans., Ⅰ, 84, 3359-3362(1988) https://doi.org/10.1039/f19888403359
  20. J. Raffi, M. H. Stevenson, M. Kent, J. M. Thiery and J. J. Belliardo,' European intercomparison on electron spin resonance identification of irradiated foodstuffs,' Int. J. Food Sci. Technol., 27, 111-124(1992) https://doi.org/10.1111/j.1365-2621.1992.tb01186.x
  21. E. M. Stewart, 'The application of ESR spectroscopy for the identification of irradiated crustacea,' Appl. Magn. Reson., 10, 375-393(1996)
  22. E. M. Stewart, 'The use of ESR spectroscopy for the detection of irradiated crustacea with particular reference to Nephrops Norvegicus(Norway Lobster),'PhD. Thesis, The Queens University of Belfast, Northern Ireland(1993)
  23. E. M. Stewart, M. H. Stevenson and R. Gray, 'Detection of irradiated in scampi tails-effects of sample preparation, irradiation dose and storage on ESR response in the cuticle,'Int. J. Food Sci. Tech., 27, 2, 125-132(1992) https://doi.org/10.1111/j.1365-2621.1992.tb01187.x
  24. J. Raffi and J. P. Agnel, 'Electron spin resonance of crustacea as an example of identification of ionized food,' Sciences Des Aliments, 10, 387-391(1990)
  25. E. M. Stewart, M. H. Stevenson, R. Gray and C. H. McMurray, 'The effect of processing treatment on the radiation-induced ESR signal in the cuticle of irradiated Norway Lobster (Nophrops Norvegicus),' Radiat. Phys. Chem., 42, 1-3, 367-370(1993) https://doi.org/10.1016/0969-806X(93)90268-Y
  26. R. Gray, 'Electron spin resonance spectroscopy for detection if irradiated food,'in New physico-chemical techniques for the characterization of complex food systems, Glasgow(United kingdom); Blackie, 86-108(1995)
  27. M. C. R. Symons,' Chemical and biochemical. Aspects of electron spin resonance spectroscopy,'Wokingham: Van Nostrand reinhold company Ltd(1978)
  28. P. Moens, F. Callens, P. Matthys and F. Maes, 'Absorption of carbonate derived molecules on the surface of carbonate containing apatites,' J. Chem. Soc., Faraday Trans., 87, 91, 3137-3141(1991) https://doi.org/10.1039/FT9918703137
  29. K. Ostrowsky, A. Dziedzic-Goctawska and W. Stachowicz, 'Stable radiation induced paramagnetic entities in tissue minerals and their use in calcified tissue research,'in : Free radicals in biology, 321-343. London: Academic press(1980)
  30. R. Gray and M. H. Stevenson, 'Effect of length of storage on the ESR signal from various bones in irradiated chicken carcasses,'Int. J. Food Sci. Technol., 25, 506-511(1990) https://doi.org/10.1111/j.1365-2621.1990.tb01109.x
  31. M. H. Stevenson and R. Gray, 'Effect of irradiation dose, storage time and temperature on the ESR signal in irradiated chicken bone,'J. Sci. Food Agric., 48, 269-274(1989) https://doi.org/10.1002/jsfa.2740480303
  32. M. F. Desrosiers and M. G. Simic, 'Postirradiation dosimetry of meat by electron spin resonance spectroscopy of bones,' J. Agric. Food Chem., 36, 601-603(1986) https://doi.org/10.1021/jf00081a049