Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지

In Vitro Germination and Propagation by Embryo Culture of Taxus cuspidata for the Taxol Production

  • Published : 2000.01.01
Download PDF
⟨ Previous Next ⟩

Abstract

To develop an efficient propagation method for yew tree, zygotic embryos were cultured under various conditions. When dissected embryos were cultured on GA$_3$ containing media, the highest germination frequency was observed on WPM medium contaning 1.0 mg/L GA$_3$. For germination of the embryos, two different conditions were compared; culturing embryos with endosperm (Method I), and 2) culturing embryos only (Method II). Maximum germination was achieved in 0.5 mg/L GA$_3$ when embryos with endosperm were cultured on the media. Of the media tested, White and WPM medium were the most suitable on germination of embryos. The abnormality of yew embryos found was observed when it cultured on GA$_3$ or culture media. About 40% of the precociously germinated embryos could be developed into full seedlings. Seedlings contained taxol in high quantity (535 $\mu\textrm{g}$/g dry weight). In vitro techniques will be sewed as a useful tool for the development of transformed root cultures and biosynthesis studies.

Keywords

References

  1. C&E Scientists mobilize to increase supply of anticancer drug taxol Borman S
  2. Plant Tissue and Cel Culture In vitro propagation of tree species Boulay M
  3. Korean Plant Tissue Culture v.20 Micropopagation of Pinus densiflora Choi MS;Lee SG;Liu JR:Pakr YG
  4. Planta Medica v.61 Taxol and related compounds in Korean native yew(Taxus cuspidata) Choi MS;Kwak SS;Liu JY;Park YG;Lee MK;An NY
  5. Proc An Assoc Cancer Res v.30 Cell culture as a means to produce taxol Cristen AA;Bland J;Gibson DM
  6. In Vitro Cell Dev Biol v.27 in vitro culture and precocious germination of Taxus embryos Flores HE;Sgringnoli PJ
  7. Exp Cell Res v.50 Nutrient requirements of suspension cultures of soybean root cells Gamberg OL;Miller RA;Ojima K
  8. Inc COngress Plant Tissue Culture Plant propagation by tissue culture George EF;Sherrington PD
  9. J. Pharm Belg v.46 Enzyme-linked immuniabsorbant assay for the detection and semi-quantitative determination of taxane diterpenoids related to taxol in Taxus spp. and tissue culture Jaziri M;Diallo BM;Vanhaeler MH;Vanhaelen-Fastre RJ;Zhiri A;Becu AG;Homes J
  10. Phytochemistry v.40 Taxol contents in the seeds of Taxus spp Kwak SS;Choi MS;Park YG;Yoo JS;Liu JR
  11. Biol Plantarun v.15 Etude en culture in vitro de la dormance embryonnaire chez. Taxus baccata C Lepage-Degivry
  12. Proc Inter Plant Prop Soc v.30 Commercially feasible micropropagation of mountain laured, klamia latifolia by use of shoot tip culture Lloyd G;McCown B
  13. Physiol Plant v.15 A revised medium for rapid growth and bioassays with tobacco tissue cultures Murashige T;Skoog F
  14. Plant Cell tissue and Organ Culture v.5 In vitro differentiation of plantlets from embryoic explants of lodgepole pine(Pinus controrta Dougl ex Loud) Patel K;Thorpe TA
  15. In: Inter. Rev Cytol Embryo culture Raghaven V;B.I. Vasil(ed.)
  16. Can J Bot v.50 Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cultures Shenck RU;Hidebrandt AC
  17. J Am Chem Soc v.93 Plant antitumor agent Ⅵ. The isolation and structure of taxol, a novel antileucokemic and antitumor agent from Taxus brevifolia Wani MC;Taylor HL;Wall ME;Coggon P;McPhail AT
  18. Growth v.7 Nutrient deficiency studies and an improved inorganic nutrient for cultivation of excised tomato roots White PR